YAN Xin-tao,WU Yun-liang,ZHA Qiao-zhen,et al.Applications and Challenges of Microbial Detection in Drinking Water by Flow Cytometry[J].China Water & Wastewater,2020,36(22 22):89-95.
Applications and Challenges of Microbial Detection in Drinking Water by Flow Cytometry
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第36卷
Number:
22 22
Page:
89-95
Column:
Date of publication:
2020-11-17
- Keywords:
- flow cytometry; drinking water; microorganism; bacteria; tap water
- Abstract:
- Flow cytometry (FCM) is an interdisciplinary technology that enables high-throughput and multi-parameter detection of suspended single cells or particles in the liquid phase. It’s particularly suitable for the detection of microorganisms with a particle size of 0.2 to 20 μm. However, in the early stage of the FCM development, the FCM had not been widely used in the rapid detection of microorganisms due to the limitations of specific binding of fluorescent dyes and instrument sensitivity. In recent years, with the development of photoelectric detection technology and new fluorescent dyes, FCM has been rapidly developed and widely used in the field of drinking water safety and security. At present, FCM can quickly and quantitatively detect the total cell concentration (TCC) to identify bacterial activity, distinguish bacteria with different nucleic acid content and size in drinking water. This review briefly discussed the basic principles, absolute counting methods, fluorescent dyes and data analysis of FCM, focusing on its recent applications in aquatic microbiology. Finally, the problems faced by FCM in the field of drinking water microbiology application are described, and the prospects for its future development are discussed.
Last Update:
2020-11-17