WU Dai-shun,YANG Xin-yi,YU Xue,et al.Effect of C/N Ratio on Microbial Metabolism and Related Functional Genes in Biological Nitrification Process[J].China Water & Wastewater,2021,37(7 7):20-26.
Effect of C/N Ratio on Microbial Metabolism and Related Functional Genes in Biological Nitrification Process
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第37卷
Number:
7 7
Page:
20-26
Column:
Date of publication:
2021-04-01
- Keywords:
- C/N ratio; nitrification process; metabolic function; nitrogen metabolism; functional genes
- Abstract:
- Ratio of carbon-nitrogen(C/N) is one of the key factors affecting the efficiency of biological denitrification. Experiments were carried out in four lab-scale sequencing batch reactors fed with synthetic wastewater with C/N ratio of 0, 5, 10 and 15 respectively. The effect of C/N ratio on microbial metabolism and related functional genes in SBR biological denitrification process was explored by means of 16S rRNA high-throughput sequencing technology. The results showed that C/N ratio had different effects on the metabolic function of microbial community. Energy metabolism, carbohydrate metabolism and amino acid metabolism were the main functions that affected the metabolic activity of microorganisms. The relative abundances of carbohydrate metabolism and energy metabolism were the highest in R0, while the relative abundances of butanoate metabolism, propanoate metabolism and nitrogen metabolism were the lowest. In addition, C/N ratio could significantly affect the number of relative functional genes in nitrogen metabolism process. When C/N ratio increased from 0 to 15, the relative abundance of related enzymes in nitrification increased at the beginning, and then decreased; the relative abundance of related enzymes in denitrification gradually increased, but the relative abundance of related enzymes in nitrogen fixation tended to decrease.
Last Update:
2021-04-01