LIU Guo-chen,ZHAO Zhi-wei,LIANG Zhi-jie,et al.Improving the Accuracy of Zebrafish Alarm through Multi-point Water Intake and Weak Voltage Stimulation[J].China Water & Wastewater,2021,37(7 7):42-48.
Improving the Accuracy of Zebrafish Alarm through Multi-point Water Intake and Weak Voltage Stimulation
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第37卷
Number:
7 7
Page:
42-48
Column:
Date of publication:
2021-04-01
- Abstract:
- In order to improve the accuracy and stability of zebrafish in actual water toxicity monitoring applications, an improved experiment was carried out. In the study, an aluminum plate with a 1 cm diameter hole was placed at 3 cm left and right of the monitoring breeding box to change the diffusion method of pollutants. At the same time, the frequency conversion voltage was added to the left and right of the aluminum plate to form a stable electric field and stimulate the zebrafish before and after the poisoning reaction. The results showed that in the multi-point water intake mode, the pollutants could diffuse into the breeding box more quickly and evenly, and contact with the zebrafish. The alarm was about 10 minutes in advance and improved the sensitivity of the alarm. A voltage of 10 V could be selected to stimulate the zebrafish after the poison exposure every 10 min, and the stimulation time was 1 min to check whether the alarm was true or false, and improved the accuracy of the alarm. Intermittent 10 V electrical stimulation was set between 0.5-1.5 h after exposure to the poison. If the speed and turnover behavior of the zebrafish was significantly lower than the blank, the alarm was considered true.
Last Update:
2021-04-01