ZENG Xiao-kang,SUN Shi-quan,GAO Yang,et al.Characteristics and Kinetics of Oil Production from Kitchen Waste Hydrolysate Fermentation[J].China Water & Wastewater,2021,37(15 15):83-88.
Characteristics and Kinetics of Oil Production from Kitchen Waste Hydrolysate Fermentation
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第37卷
Number:
15 15
Page:
83-88
Column:
Date of publication:
2021-08-01
- Keywords:
- kitchen waste hydrolysate; Saccharomyces cerevisiae; microbial oils; fermentation; kinetics
- Abstract:
- To investigate the kinetic characteristics and influencing factors of oil production from kitchen waste hydrolysate fermentation, a fermentation experiment was carried out in a 1 L fermenter, in which Saccharomyces cerevisiae As2.516 (S. cerevisiae As2.516) was used as a test strain the dosage of initial kitchen waste hydrolysate was 90%, agitation speed was 180 r/min, aeration rate was 2.5 L/min and fermentation cycle was 10 days. The initial optimal culture conditions for S. cerevisiae As2.516 were as follows: pH of 6, temperature of 30 ℃ and inoculation percentage of 10%. Based on the optimum culture condition, the highest oil yield was 4.6 g/L on the 7th day of fermentation, the biomass was 13 g/L and the oil yield rate was 36.1%. Fatty acids produced by fermentation were mainly composed of C16 and C18 fatty acids. Contents of sodium (Na+), potassium (K+), iron (Fe3+), magnesium (Mg2+) and manganese (Mn2+) in the kitchen waste hydrolysate were abundant, and there was no need to add other inorganic salts except for adding trace copper sulfate (CuSO4·5H2O, 0.1 mg/L). In addition, three fermentation kinetics models of S. cerevisiae As2.516 growth, production formation and substrate (reducing sugar) depletion were established based on Logistic and Luedeking-Piret equations, and the process of cell growth, oil synthesis and substrate (reducing sugar) consumption could be well described by these models.
Last Update:
2021-08-01