LIU Xin-yi,TANG Zhi-ye,ZHU Xiao-xi,et al.Comparison of Extracellular Polymeric Substance Extraction Methods from Activated Sludge in Sequencing Batch Reactor[J].China Water & Wastewater,2021,37(17 17):25-31.
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Comparison of Extracellular Polymeric Substance Extraction Methods from Activated Sludge in Sequencing Batch Reactor

China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU] volume: 第37卷 Number: 17 17 Page: 25-31 Column: Date of publication: 2021-09-01
Author(s):
LIU Xin-yi1; 2; TANG Zhi-ye1; 2; ZHU Xiao-xi1; 2; CHEN Qing-hua1; 2; 3; HUANG Lei1; 2; 3
(1. Key Laboratory of the Three Gorges Reservoir Region’s EcoEnvironment , Southwest University, Chongqing 400716, China; 2. College of Resources & Environment, Southwest University, Chongqing 400716, China; 3. Chongqing Engineering Research Center of Rural Cleaning, Chongqing 400716, China)
Keywords:
extracellular polymeric substance; activated sludge; extraction method; ultrasonic; cation exchange resin
Abstract:
The extraction efficiency of extracellular polymeric substances (EPS) from activated sludge in the sequencing batch reactor (SBR) by five methods, including acid extraction method, alkali extraction method, heating extraction method, ultrasonic extraction method and ultrasonic/cation exchange resin (CER) extraction method, was compared. The ultrasonic/CER extraction method was the most effective and the extraction amount of EPS reached (129.05±7.76) mg/gVSS. The total amount of EPS extracted by the acid method was the lowest. The main components of the EPS extracted from the activated sludge were protein (34.69%-63.49%), humic acid (24.93%-48.51%) and polysaccharide (9.65%-18.51%). The alkali, ultrasonic and ultrasonic/CER extraction methods had a higher extraction efficiency of the protein, and the extraction amounts were between (56.56±1.49) mg/gVSS and (57.30±3.63) mg/gVSS. The heating extraction method had the highest extraction efficiency of the humic acid with extraction amount of (55.78±5.08) mg/gVSS. The three-dimensional excitation-emission matrix spectrum showed that the fluorescence peak intensity of proteinaceous substances extracted by ultrasonic/CER method was the highest. The Fourier transform infrared spectrum showed that the main absorption peaks were closely related to the functional groups of the protein and polysaccharide, and the results were consistent with the extraction amounts of EPS. The ultrasonic/CER extraction method was recommended to extract EPS from activated sludge in SBRs after comparing the total extraction amounts and components of EPS.
Last Update: 2021-09-01