WANG Zhao-zhao,MA Jun,YIN Chun-yu,et al.Cultivation and Fast Start-up of Denitrifying Granular Sludge[J].China Water & Wastewater,2021,37(23 23):1-8.
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Cultivation and Fast Start-up of Denitrifying Granular Sludge

China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU] volume: 第37卷 Number: 23 23 Page: 1-8 Column: Date of publication: 2021-12-01
Author(s):
WANG Zhao-zhao1; 2; 3; MA Jun1; 2; 3; YIN Chun-yu1; 2; 3; WU Xin-juan1; 2; 3; ZHU Shu-hao1; 2; 3; LI Si-min1; 2; 3
(1. Hebei Technology Innovation Center of Water Pollution Control and Water Ecological Remediation, Handan 056038, China; 2. Handan Key Laboratory of Water Utilization Technology, Handan 056038, China; 3. College of Energy and Environmental Engineering, Hebei University of Engineering, Handan 056038, China)
Keywords:
denitrifying granular sludge; UASB reactor; nitrogen removal performance; physical and chemical properties
Abstract:
In the UASB reactor, flocculent activated sludge was inoculated to enhance the enrichment of denitrifying granular sludge by adjusting the substrate concentration and increasing the ascending flow rate. After 45 d operation, denitrifying granular sludge featured with clear edges, milky white and uniform particle size was rapidly cultivated. After the formation of denitrifying granular sludge, the total nitrogen removal load (NRR) and carbon removal load (CRR) reached 0.65 kg/(m3·d) and 1.97 kg/(m3·d), respectively, and the maximum specific degradation rates of NO3--N and COD were 68.90 mg/(gMLSS·d) and 182.63 mg/(gMLSS·d), respectively, indicating that the denitrifying granular sludge process exhibited a good nitrogen and carbon removal performance. In the process of denitrification granular sludge cultivation, the average value of ΔCOD/ΔNO3--N ratio was 3.35, which was always higher than the theoretical value of 2.86. The contents of protein (PN) and polysaccharide (PS) in extracellular polymer substance (EPS), and PN/PS ratio also increased gradually, which made the microorganisms to exhibit hydrophobic. At the same time,three-dimensional fluorescence spectrum (3D-EEM）analysis results showed that tryptophan, tyrosine and other proteins played important roles in accelerating the formation of denitrifying granular sludge.
Last Update: 2021-12-01