YU Li-fang,MO Peng-cheng,YANG Xiu-ling,et al.Strategy for Nitrite Oxidizing Bacteria Adapting to Low Temperature during Long-term Operation[J].China Water & Wastewater,2021,37(23 23):68-72.
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Strategy for Nitrite Oxidizing Bacteria Adapting to Low Temperature during Long-term Operation

China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU] volume: 第37卷 Number: 23 23 Page: 68-72 Column: Date of publication: 2021-12-01
Author(s):
YU Li-fang1; 2; MO Peng-cheng1; 3; YANG Xiu-ling1; LI Rong-le1; WEN Xing-mei1; PENG Dang-cong1
(1. School of Environmental and Municipal Engineering, Xi’an University of Architecture and Technology, Xi’an 710055, China； 2. Shaanxi Key Laboratory of Environmental Engineering, Xi’an University of Architecture and Technology, Xi’an 710055, China； 3. Northwest China Key Laboratory of Water Resource and Environment Ecology , Xi’an University of Architecture and Technology, Xi’an 710055, China)
Keywords:
low temperature; nitrite oxidizing bacteria (NOB); Nitrotoga; Nitrospira
Abstract:
A sequencing batch reactor(SBR) was operated continuously at 20 ℃,15 ℃ and 10 ℃ in a long-running process, and effects of temperature on reactor performance, nitrification kinetics and nitrifying bacterial community structure were analyzed. With the decrease of temperature, specific ammonia uptake rate (SAUR) decreased gradually, but specific nitrite uptake rate(SNUR) did not change significantly.Arrhenius coefficients of ammonia oxidizing bacteria(AOB) and nitrite oxidizing bacteria (NOB) during long-term operation were 1.078 and 1.007 8, respectively,indicating that NOB was more adaptable to low temperature than AOB.Quantitative PCR indicated that the abundance of AOB decreased with decreasing temperatures, while the abundance of NOB did not change significantly. Nitrotoga, a psychrophilic bacterium in NOB, increased gradually with the decrease of temperature and replaced Nitrospira to become the dominant bacterium in NOB, thus reducing the SNUR decrease caused by the decrease of Nitrospira to a certain extent. Therefore, the migration of NOB community structure from Nitrospira to Nitrotoga was the key to its adaptation to low temperature environment.
Last Update: 2021-12-01