YANGTong,TANJia-yi,WANGYan,et al.Disinfection Effect and Inactivation Mechanism of Ozone Micro-nano Bubbles on Escherichia coli[J].China Water & Wastewater,2022,38(7):1-10.
Disinfection Effect and Inactivation Mechanism of Ozone Micro-nano Bubbles on Escherichia coli
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第38卷
Number:
第7期
Page:
1-10
Column:
Date of publication:
2022-04-01
- Keywords:
- ozone micro-nano bubble; ozone micron bubble; disinfection; Escherichia coli; flow cytometry
- Abstract:
- Among the existing traditional disinfection methods in wastewater treatment plants (WWTPs), ozone (O3) inactivation has the advantages of rapid and complete inactivation rate without secondary pollution. Nevertheless, it is lack of application in domestic WWTPs at present，and the traditional aeration method produces large bubbles, poor solubility, and low mass transfer efficiency. Micro-nano bubbles (MNBs) technology can be integrated with ozone inactivation to further improve mass transfer efficiency and the overall performance of the disinfection. The results showed that the solubility of O3 micro-nano bubbles (O3-MNBs) was higher, and the dissolved O3 concentration was near 8 mg/L after aeration for 10 min. The explosion of MNBs and O3 decomposition had a synergistic mechanism to promote the production of ·OH. In the experiment of actual disinfection, 1 mg/L O3-MNBs could inactivate above 106 CFU/mL Escherichia coli (E. coli) within 1 min. In addition, under the influence of 0.2 mg/L humic acid, 2.5 mg/L O3-MNBs could still inactivate 107 CFU/mL E. coli. The results of flow cytometry analysis implied that O3-MNBs could break and lyse cells faster via thoroughgoing oxidation of esterase and DNA unwinding.
Last Update:
2022-04-01