XUFeng-ying,FANKe-feng,ZHOUJiong,et al.Nitrogen and Phosphorous Removal Performance and Microbial Community of Modified SBBR at Low Temperature[J].China Water & Wastewater,2022,38(9):82-87.
Nitrogen and Phosphorous Removal Performance and Microbial Community of Modified SBBR at Low Temperature
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第38卷
Number:
第9期
Page:
82-87
Column:
Date of publication:
2022-05-01
- Keywords:
- low temperature; SBBR; nitrogen and phosphorus removal; community
- Abstract:
- The effects of temperature on nitrogen and phosphorus removal efficiency and microbial community of a sequencing batch biofilm reactor (SBBR) under strict discharge standard were investigated, and the operational strategy for SBBR to achieve efficient nitrogen and phosphorus removal at low temperature was introduced. The temperature had a significant effect on the nitrogen removal efficiency of the system. When the temperature and COD loading rate were 10 ℃ and 0.82 kg/(m3·d), the average removal efficiencies of NH4+-N and TN were 72.4% and 70.1%, respectively, which decreased by 26.4% and 26.1% compared with those at 24 ℃. The removal efficiency of nitrogen and phosphorus was effectively enhanced by adjusting the operating condition and feeding mode of the SBBR at low temperature (10 ℃). When the operating conditions were as follows: COD loading rate of 0.41 kg/(m3·d) and cyclic time consisting of filling (6 min), anaerobic reaction (2 h), aerobic reaction (9 h), anoxic reaction (3 h, secondary filling), aerobic reaction (9 h), settling (0.8 h) and drainage (6 min), the removal efficiencies of COD, NH4+-N, TN and PO43--P increased to 95.3%, 99.2%, 95.9% and 95.1%, respectively,and the effluent quality met the class Ⅳ criteria specified in Environmental Quality Standards for Surface Water (GB 3838-2002). The 16S rRNA Amplicon high?throughput sequencing indicated that temperature had no significant effect on the microbial community composition, but had a significant effect on the relative abundance of denitrifying and phosphorous accumulating genera. When the temperature decreased from 24 ℃ to 10 ℃, the relative abundance of Nitrospira (nitrifying bacterium), Flavobacterium,Thermomonas (denitrifying bacteria) and Candidatus_Accumulibacter (phosphorous accumulating bacterium) decreased from 1.47%, 9.17%, 6.27% and 2.70% to 0.67%, 2.66%, 1.82% and 0.30%, respectively. In contrast, the relative abundance of Pseudomonas (heterotrophic nitrification and aerobic denitrification bacterium) and Dechloromonas (denitrifying phosphorus accumulating bacterium)increased from 0.07% and 0.12% to 1.05% and 1.03%, respectively.
Last Update:
2022-05-01