WANGChao-chao,ZHANGXiao-nong,LIJia-jia,et al.Effect of Carbon Sources on Performance of Partial Denitrification and Anaerobic Ammonia Oxidation Process[J].China Water & Wastewater,2023,39(3):15-24.
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Effect of Carbon Sources on Performance of Partial Denitrification and Anaerobic Ammonia Oxidation Process

China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU] volume: 第39卷 Number: 第3期 Page: 15-24 Column: Date of publication: 2023-02-01
Author(s):
WANG　Chao-chao1;  ZHANG　Xiao-nong1;  LI　Jia-jia1;  CAI　Tian-ning1;  ZHANG　Xing-xing1;  LIU　Wen-ru1; 2; 3;  LI　Xiang1; 2; 3;  WU　Peng1; 2; 3
（1. School of Environmental Science and Engineering， Suzhou University of Science and Technology， Suzhou 215009， China； 2. National and Local Joint Engineering Laboratory of Resource Utilization Technology of Municipal Sewage， Suzhou 215009， China； 3. Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment， Suzhou University of Science and Technology， Suzhou 215009， China）
Keywords:
anaerobic ammonia oxidation (Anammox);  partial denitrification;  carbon source;  COD to NO3--N ratio;  biofilm-activated sludge
Abstract:
To investigate the effect of macromolecular organic carbon sources on nitrogen removal efficiency of partial denitrification and anaerobic ammonia oxidation (PD/A) system, the biofilm-activated sludge PD/A process was rapidly started by adding glucose and new combined packing media in the Anammox continuous flow reactor, and the effects of different carbon source conditions (single carbon source and mixed carbon sources) and different COD to NO3--N ratios (COD/NO3--N) on nitrogen removal performance of the process were explored. When glucose was the sole carbon source and COD/NO3--N was 4.5, the PD/A system was successfully started on the 90th day. The removal rate of TN reached 93.89%, the TN in the effluent was 6.17 mg/L, the contribution rate of Anammox to TN removal was 95.83%, and that of denitrification to TN removal was only 4.17%. Due to the decreased utilization rate of starch and glucose mixed carbon sources by PD bacteria, the TN removal rate of PD/A system decreased to 90.53% (COD/NO3--N of 5), the TN in the effluent was 9.19 mg/L, and the contribution rate of denitrification to TN removal increased to 8.48%. The microbial high-throughput sequencing results showed that Proteobacteria was always the absolutely dominant phylum under the two carbon source conditions, and the relative abundance was always greater than 50%. Candidatus_Brocadia and Thauera, as the dominant genera in the PD/A system, played an important role in the collaborative removal of nitrogen. The relative abundance of Candidatus Brocadia and Thauera under mixed carbon sources was 2.97% and 6.52% respectively, which was slightly lower than those under single carbon source (3.41% and 6.94%). The low relative abundance of anaerobic hydrolytic acidification bacteria was the main reason for the decreased utilization of mixed carbon sources. Therefore, the paper proposed a novel biofilm-activated sludge Anammox mainstream nitrogen removal scheme for engineering application.
Last Update: 2023-02-01