SUNYu-zhu,XIAOFan,XINGMei-yan.Effect of Earthworm Active Substances in Vermifiltration on Pathogens in Excess Sludge[J].China Water & Wastewater,2023,39(9):83-90.
Effect of Earthworm Active Substances in Vermifiltration on Pathogens in Excess Sludge
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第39卷
Number:
第9期
Page:
83-90
Column:
Date of publication:
2023-05-01
- Abstract:
- The natural immune system of earthworm has a good inhibitory effect on pathogens. However, the immune function of earthworm in vermifiltration (VF) process needs further investigation. In this paper, the antibacterial ability of earthworms under stress conditions in biofilter (BF) was investigated by using a BF without earthworms as parallel control group. In addition, the changes in antibacterial ability of earthworms in VF were analyzed by using earthworms cultivated by cow dung earthworm (CDW) as control and active substances (coelomic fluid and crude lipid) as indicators, and the difference of fatty acid composition in crude lipids of two kinds of earthworms and their antibacterial protein components in coelomic fluid were analyzed and identified by GC/MS and iTRAQ. The average removal rates of 11 kinds of pathogens and indicator bacteria in VF for treatment of excess sludge was 58.0%, which was 17.8% higher than that in BF, indicating that the antibacterial ability of earthworms in VF was enhanced. Compared with CDW, there was no significant difference in antibacterial ability of earthworm fatty acids in VF, but the antibacterial ability of coelomic fluid to pathogens increased by 14.1%. Compared with CDW, earthworms in VF were subjected to stress such as continuous sludge shock and high abundance of pathogens. However, they had higher metabolic activity and stronger immune feedback, and the expression of antibacterial proteins with molecular weights in the ranges of 3-10 ku and 10-30 ku was significantly increased.
Last Update:
2023-05-01