ZHANGChen,YUJiao-jiao,LIULei,et al.Optimization of Online Solid-phase Extraction/Ultra Performance Liquid Chromatography/Mass Spectrometry for Determination of Acrylamide in Drinking Water Using Response Surface Methodology[J].China Water & Wastewater,2023,39(16):147-154.
Optimization of Online Solid-phase Extraction/Ultra Performance Liquid Chromatography/Mass Spectrometry for Determination of Acrylamide in Drinking Water Using Response Surface Methodology
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第39卷
Number:
第16期
Page:
147-154
Column:
Date of publication:
2023-08-17
- Keywords:
- drinking water; acrylamide; response surface; online solid?phase extraction; mass spectrometry
- Abstract:
- This paper developed a new method for the detection of acrylamide in drinking water based on online solid?phase extraction-ultra performance liquid chromatography-mass spectrometry optimized by response surface methodology. The optimal detection conditions were determined by extraction optimization and mass spectrometry optimization, and the technical indicators of the method were evaluated. When the solid-phase extraction column was HLB column, the enrichment mobile phase was 0.5% ammonia solution and the eluent was 0.1% formic acid solution and 95% methanol solution, the extraction recovery rate reached the highest of 80.2%. The single factor experiment and response surface curve fitting showed that the sensitivity of the instrument reached 4.83E6 when the ion source capillary voltage was 0.57 kV, the ion source temperature was 110.17 ℃, the cone hole voltage was 29.10 kV and the desolvation gas temperature was 613.42 ℃. Based on the optimal conditions, the detection limit was 0.002 9 μg/L, the limit of detection was 0.012 μg/L, the average recovery rate was 100.38%, and the relative standard deviation was 0.19%. Compared with the national standard method, this method has the advantages of short pretreatment time and high precision.
Last Update:
2023-08-17