PENGRui,MAYue,ZHANGWen-yi.Online Headspace Solid-phase Microextraction/Gas Chromatography-Mass Spectrometry for Determination of Ten Odorous Compounds in Water[J].China Water & Wastewater,2023,39(18):147-154.
Online Headspace Solid-phase Microextraction/Gas Chromatography-Mass Spectrometry for Determination of Ten Odorous Compounds in Water
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第39卷
Number:
第18期
Page:
147-154
Column:
Date of publication:
2023-09-17
- Keywords:
- odorous compounds; solid-phase microextraction; GC-MS
- Abstract:
- A headspace solid-phase microextraction/gas chromatography-mass spectrometry method was used to determine 10 microbial-derived odorous compounds in water, including diethyl disulfide, dimethyl trisulfide, 2,4-octadiene aldehyde, n-nonanol, 2-isopropyl-3-methoxypyrazine, β-cyclocitral, indole, 2,4,6-trichloroanisole, 2-methylisoborneol and geosmin. The gas chromatography ramp-up procedure, solid phase microextraction fiber head coating, dosage of sodium chloride, extraction temperature, extraction time and desorption time were optimized by single factor experiments. The 10 microbial-derived odorous compounds were well detected under the following conditions: 85 μm CAR/PDMS extraction fiber head, adding 3.5 g of sodium chloride to every 10 mL of water sample, extracting at 80 ℃ for 60 min and desorption at 250 ℃ for 5 min in the injection port. Based on the 2-methylisoborneol detection method recommended in Standard Examination Methods for Drinking Water—Part 8:Organic Indices, the temperature rise rate near the retention time of 2-methylisoborneol and 2-isobutyl-3-methoxypyrazine was adjusted to 0 ℃/min, which effectively avoided the 2-isobutyl-3-methoxy pyrazine fragments at 95 m/z at low concentration affecting the quantification of 2-methylisobornol caused by the difficult separation between 2-methylisoborneol and 2-isobutyl-3-methoxypyrazine. Under these conditions,the linearity of the 10 microbial-derived odorous compounds was good in the range of 5.0-80.0 ng/L, the detection limits were 0.05-3.26 ng/L, the spiked recoveries were between 74.3% and 128.1%, and the relative standard deviations were 1.9%-14.4%. This analytical method is simple, rapid and sensitive, and is suitable for the detection of 10 kinds of microbial-derived odorous compounds in water.
Last Update:
2023-09-17