CHENYong-yan,LüJia,ZHANGLan,et al.Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry for Determination of 135 Pesticides and Metabolites in Water[J].China Water & Wastewater,2023,39(20):143-154.
Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry for Determination of 135 Pesticides and Metabolites in Water
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第39卷
Number:
第20期
Page:
143-154
Column:
Date of publication:
2023-10-17
- Keywords:
- ultra high performance liquid chromatography tandem mass spectrometry; pesticide; source water; drinking water
- Abstract:
- This paper established a method of direct injection coupled with ultra high performance liquid chromatography tandem mass spectrometry for rapid screening and detection of 135 pesticides and metabolites in water. The samples were filtered through hydrophilic polytetrafluoroethylene（PTFE） filter membrane with aperture of 0.22 μm, directly injected into the chromatographic column in which acetonitrile and 0.05% formic acid aqueous solution were used as mobile phase for gradient elution, and separated by HSS T3 column. The samples were detected by electrospray ionization in positive and negative ion modes, and quantified by external standard method. The 135 pesticides and metabolites had good linear relationship in their respective ranges. The linear correlation coefficient r2 was greater than 0.995. The limits of detection (LOD, S/N ratio of 3) were between 0.015 and 1.5 ug/L, and the limits of quantification (LOQ, S/N ratio of 10) were from 0.05 to 5 ug/L. The target compounds were spiked at concentrations of 0.5 ug/L, 5 ug/L, and 50 ug/L, respectively. The recoveries of water samples from source water and drinking water were in the ranges of 62.9%-123.7% and 59.3%-122.3%, respectively, and the relative standard deviations (RSD, n=6) were in the ranges of 0.3%-17.4% and 0.3%-17.6%, respectively. This method solved the problem of target compounds adsorbed by filter membrane when many kinds of pesticides were directly injected. The sample pretreatment is simple, and the detection of 135 kinds of pesticides and metabolites takes only 15 min. Therefore, the method has the advantages of fast analysis, high sensitivity and good reproducibility, and is suitable for the analysis and determination of trace pesticide pollutants in source water and drinking water, which provides reliable technical support for rapid determination of pesticide pollution in water bodies.
Last Update:
2023-10-17