CHENShun-qin,GAOHuan,ZHUFa-ze,et al.Ultra-high Performance Liquid Chromatography Tandem Mass Spectrometry for Determination of Etomidate in Wastewater[J].China Water & Wastewater,2024,40(4):132-136.
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Ultra-high Performance Liquid Chromatography Tandem Mass Spectrometry for Determination of Etomidate in Wastewater

China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU] volume: 第40卷 Number: 第4期 Page: 132-136 Column: Date of publication: 2024-02-17
Author(s):
CHEN　Shun-qin1;  GAO　Huan1;  ZHU　Fa-ze1;  CHEN　Yue-meng2;  LIU Shan2;  DING　Wen-bo3;  LIU　Yu-bo1
（1. School of Forensic Medicine， Guizhou Medical University， Guiyang 550004， China； 2. Drug Identification Center， Guiyang Municipal Public Security Bureau， Guiyang 550018， China； 3. Guizhou Wanfu Xianyi Testing Technology Limited Company， Guiyang 550009， China）
Keywords:
UPLC-MS/MS;  etomidate;  wastewater
Abstract:
This paper established a method for analyzing and detecting etomidate in wastewater for the first time based on ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The target compounds in the wastewater samples were extracted by on-line solid phase extraction (on-line SPE) system with HLB Direct Connect HP solid phase extraction column (2.1 mm×30 mm, 20 μm). Then, acetonitrile, 2 mmol/L ammonium formate and 0.1% formic acid aqueous solution were employed as mobile phase for gradient elution, and the target compounds were reverse loaded to Kinetex Biphenyl column (3.0 mm×100 mm; 2.6 μm) and eventually analyzed by using electrospray ionization (ESI+) and multiple reaction monitoring (MRM) mode analysis. Etomidate had a good linear relationship in the range of 1-300 ng/L. The R2 was 0.999 7, the limit of detection (LOD) was 0.3 ng/L, and the limit of quantitation (LOQ) was 1.0 ng/L. The method was applied to detect the concentration of etomidate in the inlet of 17 wastewater treatment plants in Zunyi, and the reliability of the method was further verified. The low detection rate of etomidate in wastewater samples might be due to the fact that etomidate existed mainly in the form of etomidate acid after metabolism in body, and the detection results were consistent with the actual situation. Etomidate has been included in the second category of psychotropic drugs since October 1, 2023, and the establishment of this method provides a technical reference for the detection of etomidate.
Last Update: 2024-02-17