TANGXin,LIYan-cheng,ZHONGXiong,et al.Nitrogen Removal Performance and Microbial Degradation Mechanism of Carbon Source from Brewing Wastewater[J].China Water & Wastewater,2024,40(9):56-62.
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Nitrogen Removal Performance and Microbial Degradation Mechanism of Carbon Source from Brewing Wastewater

China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU] volume: 第40卷 Number: 第9期 Page: 56-62 Column: Date of publication: 2024-05-01
Author(s):
TANG　Xin1;  LI　Yan-cheng1; 2;  ZHONG　Xiong1;  CHEN　Chen1;  LI　Jiang1; 2;  YANG　Ai-jiang1; 2
（1. Key Laboratory of Karst Geological Resources and Environment ， College of Resources and Environmental Engineering， Guizhou University， Guiyang 550025， China； 2. Guizhou Karst Environmental Ecosystems Observation and Research Station ， Guiyang 550025， China）
Keywords:
brewing wastewater;  liquid carbon source;  biological nitrogen removal;  metagenome;  metabolic pathway
Abstract:
This paper constructed a biological denitrification system for treating low C/N ratio domestic sewage with high-concentration brewing wastewater as liquid carbon source, investigated the effects of volumetric loading rate, dissolved oxygen and air pump on/off ratio on nitrogen removal efficiency, and analyzed the microbial degradation mechanism of the system by three-dimensional fluorescence spectrum, scanning electron microscopy and metagenomics. The optimal operational parameters were as follows: the volumetric loading rate was 0.111 kgCOD/(m3·d), the dissolved oxygen was 1-2 mg/L, and the air pump on/off ratio was 3∶1. The three-dimensional fluorescence spectrum analysis showed that the system had obvious effect on the removal of dissolved microbial metabolites, and had certain degradation ability of tryptophan-like protein substances, fulvic acid-like substances, melanoid-like substances and lignocellulose-like substances. The scanning electron microscopy results showed that the shape of microorganisms was mainly spherical, rod-shaped, filamentous and helical. The dominant genera in the system were Micropruina, Propionibacterium and Nakamurella as analyzed by metagenomic technology. The annotation results based on KEGG database showed that the dominant function was metabolism, and there were 6 complete pathways related to nitrogen metabolism in the system, among which denitrification was the main pathway. In addition, there were 47 complete pathways related to carbon metabolism, among which tricarboxylic acid (TCA) cycle was the main one.
Last Update: 2024-05-01