WANGHao-long,ZHAOYu-han,ZHOULi-shan,et al.Acid Production and Microbial Community of Sludge Fermentation Enhanced by Ultrasound Combined with Bioenzymes[J].China Water & Wastewater,2024,40(11):115-120.
Acid Production and Microbial Community of Sludge Fermentation Enhanced by Ultrasound Combined with Bioenzymes
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第40卷
Number:
第11期
Page:
115-120
Column:
Date of publication:
2024-06-01
- Keywords:
- excess sludge; fermentation; acid production; ultrasound; bioenzyme; microbial community; volatile fatty acids
- Abstract:
- This paper determined the optimal sludge treatment conditions by analyzing the sludge properties, VSS removal rate, volatile fatty acids (VFAs) yield, composition and microbial community changes, so as to investigate the acid production in the fermentation of excess sludge enhanced by ultrasound combined with bioenzymes. After ultrasonic treatment, the SCOD, TN and TP in the supernatant increased significantly, the particle size of sludge decreased, and the specific surface area of sludge increased. Ultrasound combined with composite enzymes demonstrated the best performance for promoting sludge hydrolysis and acid production. The VSS removal rate reached 44.2%, and the VFAs concentration was 1 209.2 mg/L, of which the acetic acid concentration was 736.3 mg/L. The results of microbial sequencing showed that the abundance and diversity of microorganisms decreased significantly after ultrasound combined with bioenzymes (composite enzyme and lysozyme) treatment. At the phylum level, the sludge treated with composite enzyme were dominated by hydrolytic acid?producing bacteria such as Firmicutes, Proteobacteria and Bacteroidetes. At the genus level, the abundances of hydrolytic acid?producing bacteria such as Clostridium_sensu_stricto_11, Propionispira and Acetobacter were significantly different from those of the blank group.
Last Update:
2024-06-01