ZHANGXian-qing,YU Xiao-jun,ZHANGKun,et al.Establishment of Synergistic Biological Phosphorus Removal Pattern Based on Carbon Source Transformation and Its Performance[J].China Water & Wastewater,2024,40(19):1-8.
Establishment of Synergistic Biological Phosphorus Removal Pattern Based on Carbon Source Transformation and Its Performance
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第40卷
Number:
第19期
Page:
1-8
Column:
Date of publication:
2024-10-01
- Keywords:
- EBPR; SBR; carbon source transformation; Tetrasphaera; synergistic mechanism
- Abstract:
- A sequencing batch reactor (SBR) operated under alternating anaerobic/aerobic conditions was employed to investigate the effects of carbon source switching from glucose to a mixture of amino acids (glutamic acid, glycine, proline, and aspartic acid) on the performance and microbial community structure of an enhanced biological phosphorus removal (EBPR) system, and to establish a synergistic EBPR patten. The results indicated that the change in carbon source was beneficial for phosphorus removal, with an average phosphorus removal efficiency exceeding 90%. However, as the proportion of mixed amino acids increased, the ammonia nitrogen removal efficiency showed a declining trend due to the increased hydrolysis of amino acids. During the operation mid-phase, sludge bulking occurred. Nevertheless, phosphorus removal performance recovered after system adjustments. Although the amount of ammonia nitrogen removed was improved slightly, the removal efficiency did not increase. High-throughput 16S rRNA sequencing revealed that when the substrate was switched from glucose to mixed amino acids, fermentative polyphosphate-accumulating organisms (PAOs) like Tetrasphaera, and the abundance of traditional PAOs, such as Accumulibacter, significantly increased, while the abundance of nitrifying bacteria decreased. These findings were consistent with the observed phosphorus and ammonia nitrogen removal performances, suggesting that the mixed amino acids were conducive to the establishment of synergistic EBPR patten. Fermentative genera such as Tetrasphaera could biodegrade amino acids into small organic molecules, which were more easily utilized by Accumulibacter, thereby achieving synergistic phosphorus removal.
Last Update:
2024-10-01