ZHANGZe-nan,WEIYao-li,ZHOUAi-juan,et al.Pathway and Mechanism Analysis of Metronidazole Degradation through Bioelectrically Activated Anaerobic Microorganisms[J].China Water & Wastewater,2024,40(19):94-101.
Pathway and Mechanism Analysis of Metronidazole Degradation through Bioelectrically Activated Anaerobic Microorganisms
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第40卷
Number:
第19期
Page:
94-101
Column:
Date of publication:
2024-10-01
- Keywords:
- bioelectric activation; anaerobic microorganism; metronidazole; degradation path; bio?anode
- Abstract:
- Metronidazole (MNZ) is a prevalently utilized antibiotic that proves challenging to be degraded in conventional wastewater treatment processes. Therefore, with MNZ serving as the sole carbon source, the degradation performance of MNZ by bioelectrically activated anaerobic microorganisms was examined. Simultaneously, the electrochemical performance, degradation pathway and anode functional microbial community of the system were analyzed. After 150 days of targeted acclimation, the removal efficiency of MNZ could attain 74.4% within 10 days of the reaction. The maximum voltage and maximum power density were 0.072 V and 7.61 mW/m2 respectively. Additionally, the cyclic voltammetry analysis demonstrated that the oxidation potential of the anodic functional microbial community was highly similar to that of Rhodopseudomonas, indicating that electron transfer within the system might be conducted via an intermediary. The degradation products of MNZ in this system were further analyzed by LC-MS, and it was hypothesized that the possible metabolites of MNZ were 1-hydroxyethyl 2-methyl 5-amino-imidazole, (2-methyl-5-nitroimidazole-1-yl) acetic acid and 1-hydroxyethyl 2-methylimidazole. The 16S rDNA high- throughput sequencing results indicated that functional bacteria such as Truepera, Mycobacterium and Aquamicrobium were enriched in high abundance and became the dominant bacteria. The bioelectrically activated system facilitated the degradation of metronidazole through the colonization of anaerobic microorganisms, thereby providing a theoretical foundation for the investigation of the antibiotics biodegradation.
Last Update:
2024-10-01