ZHANGHuan-huan,HANLe-di,GUONing,et al.Impact of Salinity on Antibiotic Resistance Genes during Antibiotics Removal in Nitrifying Sludge System[J].China Water & Wastewater,2025,41(3):72-77.
Impact of Salinity on Antibiotic Resistance Genes during Antibiotics Removal in Nitrifying Sludge System
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第41卷
Number:
第3期
Page:
72-77
Column:
Date of publication:
2025-02-01
- Keywords:
- nitrifying sludge; antibiotics; salinity; antibiotic resistance genes (ARGs); microbial community
- Abstract:
- The nitrifying sludge process has been shown to effectively eliminate antibiotics; however, the impact of salinity on the removal of antibiotic resistance genes (ARGs) within the nitrifying sludge system remains poorly understood. In this paper, the impact of varying salinities (0, 0.5%, 1.0%, and 2.0%) on the performance of the nitrifying sludge system in treating sulfadiazine (SDZ) wastewater was investigated. Concurrently, the impact mechanisms of salinity on ARGs were examined using microbiological and statistical methods, and the microbial community composition under different salinity conditions was characterized. High salinity significantly reduced the antibiotic removal efficiency by inhibiting the activity of antibiotic-degrading bacteria, including ammonia-oxidizing bacteria and heterotrophic bacteria. Specifically, at a salinity level of 2.0%, the removal efficiency of sulfadiazine (SDZ) was only 42.3%. Furthermore, the impact of salinity on ARGs was attributed to horizontal gene transfer and alterations in microbial community composition. Elevated salinity was likely to promote the proliferation of ARGs. The highest abundance of ARGs was observed at a low salinity of 0.5%, with the abundances of sul1 and sul2 being 3.79×10-4 copies and 2.97×10-4 copies（Standardized with 16S rRNA), respectively.
Last Update:
2025-02-01