CHENQun,ZHANGYa,WANGYuan-yuan,et al.Characteristics of Carbon, Nitrogen, and Phosphorus Removal and Microbial Community Structure of a Continuous Flow AOA Process[J].China Water & Wastewater,2025,41(13):10-17.
Characteristics of Carbon, Nitrogen, and Phosphorus Removal and Microbial Community Structure of a Continuous Flow AOA Process
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第41卷
Number:
第13期
Page:
10-17
Column:
Date of publication:
2025-07-01
- Keywords:
- continuous flow AOA process; complete nitrification; denitrifying phosphorus removal; microbial community structure; high?throughput sequencing
- Abstract:
- In this study, simulated municipal wastewater was treated utilizing a continuous flow AOA reactor inoculated with residual sludge from a secondary sedimentation tank. The optimal conditions for the initiation and operational characteristics of the AOA nitrogen and phosphorus removal system were systematically explored by modulating the influent COD concentration, aeration rate, and influent flow rate. Following 70 days of continuous operation, the total nitrogen removal efficiency achieved a maximum of 60.93%. In the anaerobic zone, the polyphosphate-accumulating organisms (PAOs) and glycogen-accumulating organisms (GAOs) utilized the organic carbon sources with an effluent COD concentration of 42.20 mg/L; at the same time, PAOs completed phosphorus release, with the highest release amount reaching 23.49 mg/L. In the aerobic zone, the aerobic phosphorus uptake by PAOs exhibited considerable effectiveness, attaining a concentration of 20.70 mg/L, while the removal efficiency of NH4+-N remained consistently at 100%, facilitating the complete conversion of NH4+-N to NO3--N. In the anoxic zone, PAOs and GAOs utilized NO3--N for endogenous short-cut denitrification and phosphorus removal, achieving a NO2--N accumulation rate of 66.80%. High?throughput sequencing analysis results revealed that the relative abundance of GAOs Candidatus_Competibacter increased from 0.96% to 1.20%. Furthermore, the abundance of PAOs Candidatus_Microthrix, Hyphomicrobium, and Tetrasphaera exhibited significant increases, rising from 4.04%, 2.51%, and 3.18% to 15.36%, 5.28%, and 5.41%, respectively. This resulted in the system demonstrating stable anaerobic phosphorus release, aerobic phosphorus uptake, anoxic endogenous short-cut denitrification, and phosphorus removal efficiency.
Last Update:
2025-07-01