ZHAOZi-zheng.Ethanol Stimulation of Direct Interspecies Electron Transfer Enhancing Anaerobic Degradation of Antibiotics[J].China Water & Wastewater,2025,41(23):112-117.
Ethanol Stimulation of Direct Interspecies Electron Transfer Enhancing Anaerobic Degradation of Antibiotics
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第41卷
Number:
第23期
Page:
112-117
Column:
Date of publication:
2025-12-01
- Keywords:
- anaerobic digestion; tetracycline hydrochloride; ethanol; direct interspecies electron transfer
- Abstract:
- The anaerobic digestion efficiency of tetracycline hydrochloride (TCH) was enhanced by the addition of ethanol to the digesters. In the fourth period, during which TCH was the sole substrate, the ethanol co-digestion reactor achieved COD and TCH removal efficiencies of 62.9% and 70.6%, respectively. These values represented 21.8% and 38.4% improvement over those observed in the blank reactor, and 15.6% and 30.0% increase compared to the sodium acetate co-digestion reactor. Biological activity results demonstrated that ethanol co-digestion significantly enhanced the electron transport system (ETS) activity, specific methanogenic activity (SMA), and coenzyme F420 content in the sludge, indicating that ethanol supplementation improved microbial metabolic activity and electron transfer efficiency in the anaerobic digestion system. Microbial community analysis revealed a significant enhancement in the abundance of electroactive bacteria, specifically Geobacter and Methanothrix, in the ethanol co-digestion reactor. Therefore, it can be inferred that a direct interspecific electron transfer (DIET) mechanism likely exists between Geobacter and Methanothrix in the ethanol co-digestion reactor, playing a significant role in enhancing the removal efficiency of TCH.
Last Update:
2025-12-01