YANGJingyi,LIHongyuan,WENYuhui,et al.Screening of Heterotrophic Nitrifying Bacteria and Construction of an Enhanced Nitrogen Removal System for Mustard Tuber Wastewater[J].China Water & Wastewater,2026,42(7):65-71.
Screening of Heterotrophic Nitrifying Bacteria and Construction of an Enhanced Nitrogen Removal System for Mustard Tuber Wastewater
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第42卷
Number:
第7期
Page:
65-71
Column:
Date of publication:
2026-04-01
- Keywords:
- heterotrophic nitrification; enhanced nitrogen removal; mustard tuber wastewater; strain screening
- Abstract:
- Considering the challenges and low efficiency in the construction of nitrogen removal system for high-salt mustard tuber wastewater, this study investigated the development and performance of an enhanced heterotrophic nitrification-denitrification system for such wastewater. A heterotrophic nitrifying bacterial strain was isolated and purified from the nitrogen removal system for mustard tuber wastewater, and its physiological and biochemical properties as well as nitrogen removal performance were systematically examined. The purified bacterial solution was then introduced into the reactor to study its effects on system construction, nitrogen removal efficiency and route. The results showed that the isolated strain JZ18, identified as Pseudomonas stutzeri, was a heterotrophic nitrifying-aerobic denitrifying bacterium, which could grow under temperatures ranging from 15 ℃ to 47 ℃, pH between 5 and 9, and salinities of 10-70 g/L (calculated as NaCl). The degradation rates of COD and NH4+-N reached 23.96 mg/(L·h) and 0.90 mg/(L·h), respectively. By adding purified bacterial solution to the reactor, the enhanced heterotrophic nitrification system was constructed within 20 days. The system exhibited heterotrophic and autotrophic nitrification rates of 8.45 mg/(L·h) and 3.14 mg/(L·h), and aerobic and anoxic denitrification rates of 11.46 mg/(L·h) and 7.28 mg/(L·h), respectively. The average removal efficiencies of COD, NH4+-N and TN were 94.65%, 99.64% and 98.80%, respectively. Dominant denitrifying genera were Halomonas, Vibrio, Pseudomonas, Nitrincola, Flavobacterium, Proteiniclasticum, Phyllobacteriaceae, and Nitratireductor. The system has achieved highly efficient and stable nitrogen removal effect through the synergistic action of multiple pathways, including heterotrophic nitrification, autotrophic nitrification, aerobic denitrification and anoxic denitrification.
Last Update:
2026-04-01