LI Hui-li,HE Fu-rong,LIU Peng-cheng,et al.Optimization of Anaerobic Co-digestion Conditions of Straw and Sludge Enhanced by Exogenous Enzymes[J].China Water & Wastewater,2020,36(15 15):6-12.
Optimization of Anaerobic Co-digestion Conditions of Straw and Sludge Enhanced by Exogenous Enzymes
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第36卷
Number:
15 15
Page:
6-12
Column:
Date of publication:
2020-08-01
- Keywords:
- corn straw; municipal sludge; anaerobic digestion; cellulase; protease
- Abstract:
- Effects of exogenous enzymes on anaerobic co-digestion at medium temperature (35 ℃) were explored. In batch experiments, the mixture of straw and sludge (mass ratio=1 ∶2) was used as the substrate. Under the optimal dosage, five groups of protease and cellulase with the ratio of 1 ∶1, 1 ∶2, 2 ∶1, 1 ∶3 and 3 ∶1 were set. The gas production, methane content, ammonia nitrogen, polysaccharide, protein, volatile fatty acids (VFAs) and enzyme activity were analyzed. The maximum methane production (373.05 mL) with the TS removal efficiency of 10% and the VS removal efficiency of 43.5% was obtained when the ratio of neutral protease and cellulase was 1 ∶2, and there was no VFAs accumulation. Therefore, the enzyme ratio of 1 ∶2 could be used as the optimal dosage of exogenous enzymes for anaerobic codigestion of straw and sludge. The slurry of multi-enzyme combined reaction was determined by HPLC. It was found that the main macromolecule substances of anaerobic digestion products were biopolymer and high molecular weight humic acid.
Last Update:
2020-08-01