HUANG Si-jing,WANG Yi-jie,ZHU Bin,et al.Determination of Penicillin Residues in Water by Ultra High Performance Liquid Chromatography-High Resolution Mass Spectrometry[J].China Water & Wastewater,2021,37(2 2):112-116.
Determination of Penicillin Residues in Water by Ultra High Performance Liquid Chromatography-High Resolution Mass Spectrometry
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第37卷
Number:
2 2
Page:
112-116
Column:
Date of publication:
2021-01-17
- Keywords:
- penicillin; solidphase extraction; ultra high performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS)
- Abstract:
- High performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS) was used to determine 7 penicillin residues in water simultaneously. The water samples were enriched and purified by Oasis HLB solid phase extraction column and separated by reverse C18 chromatographic column. Then, gradient elution was implemented with 0.1% formic acid aqueous solution and acetonitrile solution as mobile phase. Finally, electrospray ion source positive ion mode was used for detection of penicillin. The correlation coefficient r of 7 penicillin compounds was greater than 0.99 and the detection limit was 1-10 ng/L in the linear range of 1.0-20.0 μg/L. Actual water sample with standard recovery rate was in the range of 91.15%-106.36%, and the relative standard deviations (RSDs) were 2.30%-8.47%, all less than 10%. The method was simple, fast and highly accurate, and could realize the determination of 7 target penicillin residues in water.
Last Update:
2021-01-17