LU Nan-nan,SHI Jin-miao,CHU Fu-min,et al.Optimization of Filtration Membrane Method for Heat-resistant Coliforms Detection in Water[J].China Water & Wastewater,2021,37(12 12):164.
Optimization of Filtration Membrane Method for Heat-resistant Coliforms Detection in Water
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第37卷
Number:
12 12
Page:
164
Column:
Date of publication:
2021-06-17
- Keywords:
- heat-resistant coliforms; fecal coliforms; filtration membrane method; enzyme substrate method
- Abstract:
- Heat-resistant coliforms are a routine test item in Standards for Drinking Water Quality (GB 5749-2006). The recommended filtration membrane detection method has the advantages of simple procedure and low cost, and is widely applied. However, the accuracy of detection results of this method is not good. Detection conditions such as detection reagent, consumables and culture conditions were optimized, and influence of humidity on the growth of heat-resistant coliforms was discussed. The culture medium, filter membrane, culture temperature and culture humidity were the key factors that affected the filter membrane method results in the detection of heat-resistant coliforms. The optimized method adopted commercial culture medium, disposable aseptic filter membrane and artificial climate incubator, and controlled the humidity at about 40%, which effectively improved the accuracy of the detection results.
Last Update:
2021-06-17