ZHAO Jing,LIU Qin,ZHANG Jing-jing,et al.Efficient Denitrification and Organic Matter Degradation Mechanism of D-A2O Process[J].China Water & Wastewater,2021,37(15 15):70-76.
Efficient Denitrification and Organic Matter Degradation Mechanism of D-A2O Process
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第37卷
Number:
15 15
Page:
70-76
Column:
Date of publication:
2021-08-01
- Keywords:
- D-A2O process; amoA; pufM; species diversity; efficient denitrification; organic matter degradation
- Abstract:
- D-A2O process is a dual-series alternating anaerobic/anoxic sewage treatment process modified by A2O process, which has significant effects on nitrogen and phosphorus removal and organic matter degradation. To investigate the reasons for efficient denitrification and organic matter degradation in D-A2O process, amoA gene and pufM gene were employed to detect and analyze community structure of denitrification and organic matter degradation microorganisms in activated sludge of D-A2O and A2O processes. At family level, 8 species of microorganisms containing amoA gene in the two processes were detected, including Nitrosomonas, Proteobacteria, Pseudomonas and Moraxella, among which Nitrosomonas and Betaproteobacteria were the dominant species. The proportion of Nitrosomonadaceae in the aerobic tank of D-A2O process (3.24%) was 1.2 times higher than that of A2O process (1.48%), the proportion of Betaproteobacteria in the aerobic tank of D-A2O process (0.21%) was twice as high as that of A2O process (0.11%), and Pseudomonadaceae and Comamonadaceae were only detected in the D-A2O process. Therefore, the D-A2O system had more abundant species of denitrifying microorganisms and higher population density. In addition, a total of 8 species of microorganisms containing pufM gene in the two processes were detected, such as Proteobacteria, Bradyrhizobiaceae, Methylobacteriaceae and so on, among which Proteobacteria and Bradyrhizobiaceae were the dominant species. The proportion of Bradyrhizobiaceae in the aerobic tank of D-A2O process (8.52%) was 1.55 times as high as that of A2O process (5.51%), and the number of Methylobacteriaceae, Sphingomonadaceae, Cetobacteraceae, Burkholderiales, Hodobacteraceae and Hromatiaceae in the D-A2O process was higher than that in the corresponding tank of the A2O process. Furthermore, analysis of α diversity and species abundance heatmap further indicated that D-A2O process had more abundant microbial species diversity and higher nitrogen removal and organic matter degradation ability.
Last Update:
2021-08-01