LIFang-xu,YANGYi-han,ZHOUXiao-hong,et al.Concentration of Enveloped Viruses in Wastewater by Centrifugal Ultrafiltration Method[J].China Water & Wastewater,2022,38(7):28-32.
Concentration of Enveloped Viruses in Wastewater by Centrifugal Ultrafiltration Method
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第38卷
Number:
第7期
Page:
28-32
Column:
Date of publication:
2022-04-01
- Keywords:
- enveloped virus; wastewater; ultrafiltration; concentration
- Abstract:
- In view of the lack of methods for efficient concentration of enveloped viruses, this paper systematically investigated the performance of centrifugal ultrafiltration method for concentration of the bacteriophage Phi6 (taking it as a model enveloped virus) in wastewater. The recovery rate of Phi6 increased with the decrease of molecular weight cut-off of the ultrafiltration cartridge, however, accompanying with the prolonged concentration time. Considering the recovery performance and time, it was recommended to select the ultrafiltration cartridge with the molecular weight cut?off of 10 ku. Under the conditions of 4 ℃, forward centrifugation of 3 000g for 28 min and backward centrifugation of 1 000g for 5 min, and 110 times of concentration ratio, the recovery rate reached (32±3.7)%.
Last Update:
2022-04-01