GEDan-dan,LIUFeng,XUGuo-dong,et al.Synergistic Properties of Sulfur Autotrophic Enhanced PHBV Heterotrophic Denitrification for Nitrogen Removal[J].China Water & Wastewater,2023,39(13):16-24.
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Synergistic Properties of Sulfur Autotrophic Enhanced PHBV Heterotrophic Denitrification for Nitrogen Removal

China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU] volume: 第39卷 Number: 第13期 Page: 16-24 Column: Date of publication: 2023-07-01
Author(s):
GE　Dan-dan1;  LIU　Feng1; 2;  XU　Guo-dong3;  ZHANG　Xue-zhi1;  FENG　Zhen1;  CHEN　Ke-xiu1
（1. School of Environmental Science and Engineering， Suzhou University of Science and Technology， Suzhou 215009， China； 2. Municipal Wastewater Utilization Technology National Local Joint Engineering Laboratory， Suzhou 215009， China； 3. Suzhou Zhongsheng Environmental Remediation Limited Company， Suzhou 215009， China）
Keywords:
solid organic carbon source;  polyhydroxybutyrate;  thiosulfate;  heterotrophic denitrification;  sulfur autotrophic denitrification;  high-throughput sequencing
Abstract:
Thiosulfate was introduced as an electron donor for autotrophic denitrification to enhance nitrogen removal in a denitrifying biofilter packed with polyhydroxybutyrate (PHBV). This paper constructed two sets of denitrifying biofilter packed with PHBV (R1 and R2), and investigated the synergistic effect of adding thiosulfate to enhance nitrogen removal performance according to different hydraulic retention time (HRT). When the nitrate nitrogen in influent was 50 mg/L, and the HRTs of R1 and R2 were shortened from 1.0 h and 1.5 h to 0.5 h and 0.75 h respectively, the nitrate nitrogen in effluent increased to 26.18 mg/L and 18.78 mg/L respectively accompanied with the accumulation of nitrite nitrogen. The removal rate of nitrate nitrogen gradually increased after adding 25 mg/L, 50 mg/L and 75 mg/L thiosulfate to the two reactors, respectively. When the HRT was 0.75 h and the thiosulfate dosage was 50 mg/L, the denitrification synergistic effect was significant, the nitrate nitrogen removal rate reached 100%, the effluent pH was between 6.8 and 7.4, and the SO42--S concentration was less than 250 mg/L. Scanning electron microscopy revealed that numerous voids appeared on the surface of PHBV under the action of microbial hydrolysis, which provided habitats for the adhesion and growth of microorganisms. High?throughput sequencing results showed that the functional bacterial genera in the two reactors mainly included Thiobacillus, Comamonadaceae and Thermomonas.
Last Update: 2023-07-01