ZHUANGYan-hua,TANGYan-hua,LUOZhi-yao,et al.Rapid Start?up of Anammox-MBR Process and Its Stable Operation[J].China Water & Wastewater,2025,41(5):72-80.
Rapid Start?up of Anammox-MBR Process and Its Stable Operation
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第41卷
Number:
第5期
Page:
72-80
Column:
Date of publication:
2025-03-01
- Keywords:
- anaerobic ammonia oxidation (Anammox); membrane bioreactor (MBR); rapid start-up; enzyme activity; microbial community composition
- Abstract:
- Anaerobic ammonia oxidation (Anammox) represents an innovative and highly efficient nitrogen removal process that also offers significant energy savings. However, the long start-up time and challenges in achieving stable operation are primary obstacles hindering its widespread engineering application. Therefore, a strategy for the rapid start-up and stable operation of an Anammox process coupled with membrane bioreactor (MBR) was proposed. A pilot-scale continuous-flow reactor was employed, utilizing simulated wastewater as the media. The reactor was seeded with anaerobic sludge from a sewage treatment plant, operating at temperature of (32.1±1.6) ℃ and pH range of 7.40 to 8.75. By implementing continuous stirring and gradually increasing the media concentration, the start-up period was successfully reduced to 135 days. When the system operated stably, the average removal rates of NH4+-N and NO2--N reached 95.61% and 92.17%, respectively. By stepwise increasing the media concentration and shortening the hydraulic retention time (HRT), the total nitrogen volumetric removal rate (NRR) was enhanced to as high as 1.36 kg/(m3·d). Consequently, the reactor demonstrated excellent denitrification performance. Three Anammox bacterial genera, namely Candidatus_Brocadia, Candidatus_Jettenia, and Candidatus_Kuenenia, were successfully cultured following a domestication process. The total abundance of the three bacterial genera on the surface of the membrane module and within the granular sludge reached 20.78% and 22.79%, respectively. This significant enrichment in distinct ecological niches enhanced the synergistic nitrogen removal efficiency among various Anammox functional bacteria.
Last Update:
2025-03-01