ZhangYuyan,LiuWenlong,YueChenhao,et al.Advanced Nitrogen Removal Performance and Microbial Functional Characteristics in a Glucose-fed SAED System[J].China Water & Wastewater,2026,42(13):9-17.
Advanced Nitrogen Removal Performance and Microbial Functional Characteristics in a Glucose-fed SAED System
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第42卷
Number:
第13期
Page:
9-17
Column:
Date of publication:
2026-07-01
- Keywords:
- Anammox; endogenous denitrification; advanced nitrogen removal; glucose; microbial community evolution; carbon and nitrogen metabolic pathways
- Abstract:
- To achieve stable and advanced nitrogen removal in the anaerobic ammonium oxidation (Anammox) process for wastewater, an Anammox coupled with endogenous denitrification (glucose-fed SAED) nitrogen removal process was constructed using glucose as the electron donor. The advanced nitrogen removal performance of the system, the succession of microbial communities, and the carbon and nitrogen metabolism pathways were investigated. Results showed that the total inorganic nitrogen removal efficiency in the glucose-fed SAED remained at (95.0±1.8)% during long-term operation (476 days), achieving long-term stable advanced nitrogen removal performance. Nitrogen balance analysis indicated that Anammox reaction [(89.7±2.8)%] was the main nitrogen removal pathway, which combined with endogenous denitrification [(10.3±2.8)%] to achieve advanced nitrogen removal. High-throughput data indicated that glucose addition significantly altered the microbial community structure of the SAED system, and Candidatus Brocadia (22.2%) was positively correlated with Denitratisoma (13.5%) and gradually became the dominant genus with cross-feeding interactions over the long-term operation. Furthermore, the carbon and nitrogen metabolic pathways analysis indicated that glycolysis and the tricarboxylic acid cycle provided sufficient electrons and ATP for anoxic nitrogen metabolism. Meanwhile, diverse nitrogen metabolic pathways and highly abundant functional genes such as partial denitrification (narG/H/I and napA/B) and Anammox functional genes (hzsA/B/C and hdh) ensured the long-term stability of the system.
Last Update:
2026-07-01