[1]王翠翠,李艳苹,刘小骐,等.HPLC与ICP-MS联用测定海水中5种硒形态[J].中国给水排水,2018,34(22):111-115.
　WANG Cui-cui,LI Yan-ping,LIU Xiao-qi,et al.Determination of 5 Selenium Species in Seawater by Hyphenated Technique of High Performance Liquid Chromatography（HPLC） and Inductivity Coupled Plasma Mass Spectrometry[J].China Water & Wastewater,2018,34(22):111-115.
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HPLC与ICP-MS联用测定海水中5种硒形态

中国给水排水[ISSN:1000-4062/CN:12-1073/TU] 卷: 第34卷 期数: 2018年22期 页码: 111-115 栏目: 分析与监测 出版日期: 2018-11-17
Title:
Determination of 5 Selenium Species in Seawater by Hyphenated Technique of High Performance Liquid Chromatography（HPLC） and Inductivity Coupled Plasma Mass Spectrometry
文章编号:
1000-4602(2018)22-0111-05
作者:
王翠翠, 李艳苹, 刘小骐, 潘献辉
(国家海洋局 天津海水淡化与综合利用研究所, 天津 300192)
Author(s):
WANG Cui-cui, LI Yan-ping, LIU Xiao-qi, PAN Xian-hui
(The Institute of Seawater Desalination and Multipurpose Utilization, SOA<Tianjin>, Tianjin 300192, China)
关键词:
高效液相色谱;  电感耦合等离子体质谱;  海水;  硒形态分析
Keywords:
high performance liquid chromatography;  inductively coupled plasma mass spectrometry;  seawater;  selenium speciation analysis
分类号:
TU991
文献标志码:
C
摘要:
研究并建立了高效液相色谱与电感耦合等离子体质谱联用(HPLC-ICP-MS)测定海水中5种硒形态的分析方法。采用Hamilton PRP-X100分析柱,在流动相为30 mmol/L柠檬酸、pH为4.0的条件下,8 min内即可完全分离5种硒形态。在色谱进样量为100 μL,质谱采用He碰撞池模式下,对SeCys2、MeSeCys、SeUr、SeMet和SeO2-4的检出限分别为0.42、0.70、0.65、1.63和0.31 μg/L,线性相关系数均大于0.998。该方法简便、快速、灵敏度高,可用于海水中硒形态的测定。
Abstract:
A method for determination of 5 selenium species in seawater by hyphenated technique of high performance liquid chromatography and inductivity coupled plasma mass spectrometry(HPLC-ICP-MS)was established. The separation of 5 selenium species was operated by the Hamilton PRP-X100 analysis column within 8 min with mobile phase of 30 mmol/L citrate and pH 4.0. When the injection volume was 100 μL and He collision cell mode was used in mass spectrometry, the detection limit of SeCys2, MeSeCys, SeUr, SeMet and SeO2-4 were found to be 0.42, 0.70, 0.65, 1.63 and0.31 μg/L, respectively. Moreover, all of the linear correlation coefficients were more than 0.998. The method was simple, rapid and with remarkably high sensitivity, which could be developed for the determination of selenium species in seawater.

参考文献/References:


[1] Mazan S,Gilon N,Crétier J L,et al. Inorganic selenium speciation using HPLC-ICP-hexapole collision/reaction cell-MS [J]. J Anal At Spectrom,2002,17(4):366-370.
[2] 曲韵笙,朱连勤,孔涛. 微量元素硒的研究进展[J]. 微量元素与健康研究,2006,23(6):68-69. Qu Yunsheng,Zhu Lianqin,Kong Tao. The advanced research of trace selenium[J]. Studies of Trace Elements and Health,2006,23(6):68-69(in Chinese).
[3] 邱建华,王秋泉,黄本立. 硒形态分析研究进展[J]. 光谱学与光谱分析,2006,26(9):1692-1701. Qiu Jianhua,Wang Qiuquan,Huang Benli. New approaches to selenium speciation[J]. Spectroscopy and Spectral Analysis,2006,26(9):1692-1701(in Chinese).
[4] Zheng J,Shibata Y,Tanaka A. Study of the stability of selenium compounds in human urine and determination by mixed ion-pair reversed-phase chromatography with ICP-MS detection[J]. Anal Bioanal Chem,2002,374(2):348-353.
[5] Gammelgaard B,Bendahl L,Sidenius U,et al. Selenium speciation in urine by ion-pairing chromatography with perfluorinated carboxylic acids and ICP-MS detection[J]. J Anal At Spectrom,2002,17(6):570-575.
[6] Kotrebai M,Tyson J F,Block E,et al. High-performance liquid chromatography of selenium compounds utilizing perfluorinated carboxylic acid ion-pairing agents and inductively coupled plasma and electrospray ionization mass spectrometric detection[J]. J Chromatogr A,2000,866:51-63.
[7] 李晶,孔维恒,高峰,等. HPLC-ICP-MS法分析测定富硒保健品中的硒形态[J]. 现代仪器,2012,18(3):36-39. Li Jing,Kong Weiheng,Gao Feng,et al. Determination of selenium species in health care products by HPLC-ICP-MS [J]. Modern Instruments,2012,18(3):36-39(in Chinese).
[8] 王欣,幸苑娜,陈泽勇,等. 高效液相色谱-电感耦合等离子体质谱法检测富硒食品中6种硒形态[J]. 分析化学,2013,41(11):1669-1674. Wang Xin,Xing Yuanna,Chen Zeyong,et al. Determination of 6 selenium species in selenium-enriched food by hyphenated technique of high performance liquid chromatography-inductively coupled plasma mass spectrometry[J]. Chinese Journal of Analytical Chemistry,2013,41(11):1669-1674(in Chinese).
[9] 铁梅,方禹之,孙铁彪,等. HPLC-ICP-MS 联用技术在富硒金针菇硒的形态分析中的应用[J]. 高等学校化学学报,2007,28(4):635-639. Tie Mei,Fang Yuzhi,Sun Tiebiao,et al. Application of HPLC-ICP-MS in speciation analysis of selenium in selenized flammulina velutipes[J]. Chemical Journal of Chinese Universities,2007,28(4):635-639(in Chinese).
[10] 喻宏伟,陈春英,高愈希,等. 高效液相色谱-电感耦合等离子体质谱法分析生物样品中硒的化学形态[J]. 分析化学,2006,34(6):749-753. Yu Hongwei,Chen Chunying,Gao Yuxi,et al. Chemical speciation analysis of selenium in biological samples by a hyphenated technique of high performance liquid chromatography-inductively coupled plasma mass spectrometry [J]. Chinese Journal of Analytical Chemistry,2006,34(6):749-753(in Chinese).
[11] Zheng J,Ohata M,Furuta N,et al. Speciation of selenium compounds with ion-pair reversed-phase liquid chromatography using inductively coupled plasma mass spectrometry as element-specific detection[J]. J Chromatogr A,2000,874(1):55-64.

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备注/Memo

作者简介:王翠翠(1989- ), 女, 河北邯郸人, 博士, 工程师, 从事水质检测, 研究方向为海水及苦咸水分析。 E-mail:pig313716@126.com
收稿日期:2018-06-20

更新日期/Last Update: 2018-12-10