HANWen-jie,ZHOUJia-zhong,LIUYan,et al.Start-up of Pure MBBR Process for Micro-polluted Water Treatment[J].China Water & Wastewater,2022,38(7):19-27.
Start-up of Pure MBBR Process for Micro-polluted Water Treatment
China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU]
volume:
第38卷
Number:
第7期
Page:
19-27
Column:
Date of publication:
2022-04-01
- Keywords:
- pure MBBR; suspended carrier; micro?polluted water; nitrification; biomass; biofilm thickness; high throughput sequencing
- Abstract:
- A two-stage pure MBBR was applied to treat micro-polluted river water. The nitrification performance of biofilm during start-up was investigated, and the changes in biofilm thickness, biomass and microbial community were analyzed simultaneously. The reactor was directly started at extremely low temperature in winter by feeding raw water without sludge inoculation, and the ammonia nitrogen in effluent reached the standard stably after 10 days of system debugging. The ammonia nitrogen concentrations in the effluent of the first and second stage MBBR were (1.35±0.38) mg/L and (0.43±0.15) mg/L, and the nitrification loads of the first and second stage MBBR were (0.182±0.026) kg/(m3·d) and (0.066±0.020) kg/(m3·d), respectively. The ammonia nitrogen removal efficiency of the system reached (88.98±3.03)%. At the same time, the system had a certain COD removal capacity. During the start-up process, the nitrification load increased and reached stability on day 14, and the biomass of the biofilm tended to be stable on day 28. The biomass of the first and second stage MBBR was (2.66±0.36) g/m2 and (2.14±0.19) g/m2, respectively, and the thickness of the biofilm reached (197±23) μm and (157±17) μm, respectively. The biofilm load had a certain redundancy and could resist the impact of the influent load. The species richness of biofilm basically stabilized on day 21, and the species richness and distribution uniformity of the first stage biofilm were higher than those of the second stage biofilm, indicating that the former had higher species diversity. The dominant bacterial genera of the biofilm were Nitrospira, Hypomicrobium, Nitrosomonas, Kouleothrix, Pedomicrobium, Pedobacter, etc. Among them, the relative abundance of Nitrospira in the biofilm from the first and second stage MBBR was 8.48%-13.60% and 6.48%-9.27%, respectively, and the relative abundance of Nitrosomonas was 2.89%-5.64%, 0.00%-3.48%, respectively. The presence of bacterial genera such as Hyphomicrobium and Pedomicrobium might be related to the transformation of aromatic hydrocarbon DOM in the influent.
Last Update:
2022-04-01