RANZhi-lin,MAOZhu,XIANGHui-qiang,et al.Mechanism of Bacillus subtilis Inactivated by Ultraviolet Light-emitting Diode and Chlorine[J].China Water & Wastewater,2022,38(11):43-48.
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Mechanism of Bacillus subtilis Inactivated by Ultraviolet Light-emitting Diode and Chlorine

China Water & Wastewater[ISSN:1000-4062/CN:12-1073/TU] volume: 第38卷 Number: 第11期 Page: 43-48 Column: Date of publication: 2022-06-01
Author(s):
RAN　Zhi-lin1; 2; 3;  MAO　Zhu3; 4;  XIANG　Hui-qiang1; 2;  LI　Shao-feng3;  NIE　Jin-xu4;  WANG　Zhi-hong4
（1. Institute of Innovational Education Research， Shenzhen Institute of Information Technology，Shenzhen 518172， China； 2. School of Traffic and Environment， Shenzhen Institute of Information Technology， Shenzhen 518172， China； 3. Shenzhen Key Laboratory of Industrial Water Saving and Municipal Sewage Reclamation Technology， Shenzhen Polytechnic， Shenzhen 518055， China；4. School of Civil and Transportation Engineering， Guangdong University of Technology， Guangzhou 510006， China）
Keywords:
ultraviolet light-emitting diode;  chlorine;  Bacillus subtilis;  inactivation mechanism
Abstract:
Bacillus subtilis was inactivated by ultraviolet light-emitting diode (UV-LED) combined with sodium hypochlorite, and the inactivation performance of UV-LED and chlorine alone was analyzed. Synergistic effect existed in the UV-LED/chlorine system, and the inactivation performance was much better than that of UV-LED and chlorine alone. Protein dissolution, dark repair and cell surface structure showed that the produced free radicals damaged the outer structure of the cell, leading to the entry of hypochlorous acid molecules and hypochlorite ions into the cells, while the damaged cells were more susceptible to UV penetration. The combination of the two actions caused DNA damage and ultimately inactivated the bacteria. In addition, the contributions of UV-LED, hydroxyl radical, chlorine, chlorine radical and hypochlorous acid to UV-LED/chlorine inactivation were evaluated, and it was found that the contribution of UV-LED and hydroxyl radical was larger.
Last Update: 2022-06-01