DENGYong-chun,CAOJiang-ling,WUPei-rong,et al.Impact of Mixing Effect in Pre-anoxic Phase on Stability of Aerobic Granular Sludge in Treating Wastewater with Low Carbon to Nitrogen Ratio[J].China Water & Wastewater,2025,41(9):8-16.
缺氧条件对低碳氮比废水好氧颗粒污泥稳定性的影响
- Title:
- Impact of Mixing Effect in Pre-anoxic Phase on Stability of Aerobic Granular Sludge in Treating Wastewater with Low Carbon to Nitrogen Ratio
- Keywords:
- aerobic granular sludge; low carbon to nitrogen ratio wastewater; anoxic phase; external circulation; stability
- 摘要:
- 好氧颗粒污泥稳定性低是阻碍其应用的主要问题。为揭示好氧颗粒污泥系统前置缺氧段混合效果对好氧颗粒稳定性的影响，在SBR反应器中接种冷藏6个月的好氧颗粒污泥，将碳氮负荷（COD/NH4+-N）从200/40 mg/L提升至600/150 mg/L，通过施加和不施加外循环操作改变缺氧段混合效果，考察好氧颗粒污泥系统处理性能、颗粒稳定性及微生物种群结构变化。结果表明，接种的好氧颗粒污泥恢复活性后，当碳氮负荷为400/50 mg/L时未开启外循环操作，系统对COD、NH4+-N和TN的去除率分别为95%、99%和20%；当碳氮负荷升至400/80和400/100 mg/L时开启外循环操作，虽然好氧颗粒污泥受反硝化气态氮冲击导致沉淀性能变差，SVI30在50~150 mL/g之间波动，系统对COD和NH4+-N的去除率仍能够分别保持在95%和99%，但是TN去除率提升至50%；而当碳氮负荷升至600/150 mg/L时再次关闭外循环操作后，好氧颗粒结构变得疏松分散，平均粒径增大至791 μm，SVI30恶化为175 mL/g，虽然COD去除率仍能保持在95%左右，但NH4+-N和TN去除率分别降低至50%和6%。高通量测序分析表明，缺氧段施加外循环操作后，好氧颗粒微生物多样性和丰富度增加，主要优势菌种为反硝化菌，主要包括Thauera和 Rhodobacter。在处理低碳氮比废水时在缺氧段施加外循环操作，能够通过增强混合效果提高系统反硝化性能，富集大量反硝化菌，从而增强好氧颗粒污泥稳定性。
- Abstract:
- Low stability of aerobic granular sludge(AGS) in long-term operation is the main problem hindering its further application in practice. To investigate the impact of the mixing effect in pre- anoxic phase on the stability of AGS, a sequencing batch reactor (SBR) inoculated with AGS refrigerated for 6 months was set up with increased carbon and nitrogen load (C/N) from 200/40 mg/L to 600/150 mg/L in this study. The mixing effect of the pre-anoxic phase was adjusted by adding and not adding an external circulation operation. The properties of the AGS, such as the performance, stability, and microbial community structure, were examined. The results showed that when the C/N was 400/50 mg/L with no external circulation, the removal rate of COD, NH4+-N and TN were 95%, 99%, and 20%, respectively, after the seeded AGS revived. When the C/N was increased to 400/80 mg/L and 400/100 mg/L with external circulation, COD and NH4+-N removal rate could still maintain at 95% and 99%, respectively, but the TN removal rate greatly increased to 50%, although the settling ability of the AGS deteriorated due to the mass production of gaseous nitrogen from denitrification, with SVI30 fluctuating between 50 mL/g and 150 mL/g. However, when the C/N was increased to 600/150 mg/L with no external circulation, NH4+-N and TN removal rate decreased to 50% and 6%, respectively, although COD removal rate could still maintain at about 95%. At the same time, the physical structure of AGS became loose and dispersed with the average particle size increased to 791 μm and SVI30 deteriorated to 175 mL/g. High-throughput sequencing analysis showed that the microbial diversity and richness of aerobic particles increased with the external circulation in the anoxic phase, and the main genus were denitrifying bacteria, mainly including Thauera and Rhodobacter. These findings demonstrated that the external circulation operation in anoxic phase can improve the denitrification performance of AGS system and enrich a large number of denitrifying bacteria by enhancing the mixing effect, thereby enhancing its stability .
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