WANGBo.Performance and Mechanism of Heterotrophic-Autotrophic Denitrification Enhanced by Iron Humate[J].China Water & Wastewater,2026,42(11):78-82.
腐殖酸铁强化异养-自养反硝化性能及机理
- Title:
- Performance and Mechanism of Heterotrophic-Autotrophic Denitrification Enhanced by Iron Humate
- Keywords:
- humic acid; ferrous ion; heterotrophic-autotrophic denitrification; electron transfer; EPS secretion
- 摘要:
- 采用腐殖酸（HA）与亚铁离子（Fe2+）形成的络合物腐殖酸铁（Fe-HA）作为功能材料，探究其对异养-自养反硝化体系的强化脱氮性能。将Fe-HA投加量作为变量设置批次实验，浓度梯度为0、30、50、70、90 mg/L，考察硝酸盐浓度的变化；并测定胞外聚合物（EPS）、电子传递系统活性（ETSA）、三磷酸腺苷（ATP）和细胞色素c等指标的变化，揭示Fe-HA对异养-自养反硝化的强化机理。结果表明，Fe-HA的最佳投加量为70 mg/L，体系对NO3--N的去除率可达96.6%，为空白对照组的1.2倍。同时，Fe-HA可促进EPS的分泌量达到15.77 mg/gVSS，ETSA、ATP和细胞色素c含量相比其他投加量均有所提高。可见，在适宜投加量下，Fe-HA可以加速微生物之间的电子传递过程，提升细胞的代谢活性和微生物之间的聚集性，从而提升脱氮性能。
- Abstract:
- Humic acid (HA) complexed with ferrous ions (Fe2+) to form iron humate (Fe-HA) was used as a functional material to investigate its denitrification performance in a heterotrophic-autotrophic denitrification system. Batch experiments were conducted with Fe-HA dosage as the variable, and the concentration gradient was set at 0 mg/L, 30 mg/L, 50 mg/L, 70 mg/L, and 90 mg/L to examine the changes in nitrate concentration. Additionally, the changes in indicators such as extracellular polymeric substances (EPS), electron transfer system activity (ETSA), adenosine triphosphate (ATP), and cytochrome c were measured to reveal the mechanism by which Fe-HA accelerates heterotrophic-autotrophic denitrification. The results showed that the optimal Fe-HA dosage was 70 mg/L, at which the NO3--N removal rate reached 96.6%, which was 1.2 times that of the blank control group. At the same time, Fe-HA promoted EPS secretion to 15.77 mg/gVSS, and the levels of ETSA, ATP, and cytochrome c were higher than those at other dosages. These findings indicated that at an appropriate dosage, Fe-HA could accelerate the electron transfer process between microorganisms, enhance cellular metabolic activity and microbial aggregation, and thereby improved nitrogen removal performance.
相似文献/References:
[1]王轶,陈卫,耿楠楠.基于TiO2/石墨烯的光催化材料降解腐殖酸[J].中国给水排水,2020,36(9):83.
[2]魏永,吴宏.纳滤处理太湖水的膜污染中试研究[J].中国给水排水,2021,37(7):66.
WEI Yong,WU Hong.A Pilot Study on Membrane Fouling in the Treatment of Taihu Lake Water by Nanofiltration[J].China Water & Wastewater,2021,37(11):66.
[3]王晓云,付爱民.二氧化氯预氧化生成无机消毒副产物的研究[J].中国给水排水,2021,37(23):34.
WANG Xiao-yun,FU Ai-min.Formation of Inorganic Disinfection By-products during Chlorine Dioxide Pre-oxidation[J].China Water & Wastewater,2021,37(11):34.
[4]刘梓晶,李红岩,王新,等.荧光光谱-KNN算法用于腐殖酸种类识别和定量[J].中国给水排水,2022,38(23):56.
LIUZi-jing,LIHong-yan,WANGXin,et al.Identification and Quantification of Humic Acid Based on K-Nearest Neighbor Algorithm Combined with Fluorescence Spectra[J].China Water & Wastewater,2022,38(11):56.