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葡萄糖型SAED系统深度脱氮性能及微生物功能解析

中国给水排水[ISSN:1000-4062/CN:12-1073/TU] 卷: 第42卷 期数: 2026年第13期 页码: 9-17 栏目: 出版日期: 2026-07-01
Title:
Advanced Nitrogen Removal Performance and Microbial Functional Characteristics in a Glucose-fed SAED System
作者:
张瑜艳1,2， 刘文龙1,2， 岳晨浩1,2， 刘志刚3， 李军1,2
（1.浙江工业大学 环境学院，浙江 杭州 310014；2.浙江省工业污染微生物控制技术重点实验室，浙江 杭州 310014；3.宁波市水务环境集团股份有限公司，浙江 宁波 315041）
Author(s):
Zhang　Yuyan1，2， Liu　Wenlong1，2， Yue　Chenhao1，2， Liu　Zhigang3， Li　Jun1，2
（1. College of Environment， Zhejiang University of Technology， Hangzhou 310014， China； 2. Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province， Hangzhou 310014， China； 3. Ningbo Water & Environment Group Co. Ltd.， Ningbo 315041， China）
关键词:
厌氧氨氧化;  内源反硝化;  深度脱氮;  葡萄糖;  菌群结构演变;  碳、氮代谢路径
Keywords:
Anammox;  endogenous denitrification;  advanced nitrogen removal;  glucose;  microbial community evolution;  carbon and nitrogen metabolic pathways
摘要:
为实现污水厌氧氨氧化工艺的稳定深度脱氮，以葡萄糖为电子供体构建厌氧氨氧化耦合内源反硝化（葡萄糖型SAED）脱氮工艺，考察了系统的深度脱氮性能、微生物群落演替规律以及碳和氮代谢路径。结果表明，葡萄糖型SAED系统在476 d的运行过程中对总无机氮的去除率保持在（95.0±1.8）%，取得了长期稳定的深度脱氮效果。氮素平衡分析表明，厌氧氨氧化反应［（89.7±2.8）%］为主要脱氮途径，协同内源反硝化［（10.3±2.8）%］实现深度脱氮。高通量测序数据表明，葡萄糖的投加虽然显著改变了SAED系统的微生物群落结构，但在长期运行中Candidatus Brocadia（22.2%）和Denitratisoma（13.5%）呈正相关关系并逐渐成为优势互作菌属。此外，碳和氮代谢路径分析表明，糖酵解和三羧酸循环为缺氧氮代谢提供了充足的电子和腺苷三磷酸（ATP），同时多样的氮代谢路径和高丰度的功能基因，如短程反硝化基因（narG/H/I、napA/B）和厌氧氨氧化基因（hzsA/B/C、hdh），保障了系统的长期稳定。
Abstract:
To achieve stable and advanced nitrogen removal in the anaerobic ammonium oxidation (Anammox) process for wastewater, an Anammox coupled with endogenous denitrification (glucose-fed SAED) nitrogen removal process was constructed using glucose as the electron donor. The advanced nitrogen removal performance of the system, the succession of microbial communities, and the carbon and nitrogen metabolism pathways were investigated. Results showed that the total inorganic nitrogen removal efficiency in the glucose-fed SAED remained at (95.0±1.8)% during long-term operation (476 days), achieving long-term stable advanced nitrogen removal performance. Nitrogen balance analysis indicated that Anammox reaction [(89.7±2.8)%] was the main nitrogen removal pathway, which combined with endogenous denitrification [(10.3±2.8)%] to achieve advanced nitrogen removal. High-throughput data indicated that glucose addition significantly altered the microbial community structure of the SAED system, and Candidatus Brocadia (22.2%) was positively correlated with Denitratisoma (13.5%) and gradually became the dominant genus with cross-feeding interactions over the long-term operation. Furthermore, the carbon and nitrogen metabolic pathways analysis indicated that glycolysis and the tricarboxylic acid cycle provided sufficient electrons and ATP for anoxic nitrogen metabolism. Meanwhile, diverse nitrogen metabolic pathways and highly abundant functional genes such as partial denitrification (narG/H/I and napA/B) and Anammox functional genes (hzsA/B/C and hdh) ensured the long-term stability of the system.

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更新日期/Last Update: 2026-07-01