[1]徐凤英,樊科峰,周炯,等.低温下改良SBBR脱氮除磷效能及微生物种群研究[J].中国给水排水,2022,38(9):82-87.
　XUFeng-ying,FANKe-feng,ZHOUJiong,et al.Nitrogen and Phosphorous Removal Performance and Microbial Community of Modified SBBR at Low Temperature[J].China Water & Wastewater,2022,38(9):82-87.
点击复制

低温下改良SBBR脱氮除磷效能及微生物种群研究

中国给水排水[ISSN:1000-4062/CN:12-1073/TU] 卷: 第38卷 期数: 2022年第9期 页码: 82-87 栏目: 出版日期: 2022-05-01
Title:
Nitrogen and Phosphorous Removal Performance and Microbial Community of Modified SBBR at Low Temperature
作者:
徐凤英1， 樊科峰1， 周炯1， 龚本洲1,2， 孟红1， 和雪杰1， 周健1
（1.重庆大学 三峡库区生态环境教育部重点实验室，重庆 400045；2.长江勘测规划设计研究有限责任公司，湖北 武汉 430010）
Author(s):
XU　Feng-ying1， FAN　Ke-feng1， ZHOU　Jiong1， GONG　Ben-zhou1，2， MENG　Hong1， HE　Xue-jie1， ZHOU　Jian1
（1. Key Laboratory of Three Gorges Reservoir Region’s Eco-Environment ， Chongqing University， Chongqing 400045， China； 2. Changjiang Institute of Survey， Planning， Design and Research， Wuhan 430010， China）
关键词:
（1.重庆大学 三峡库区生态环境教育部重点实验室; 重庆 400045; 2.长江勘测规划设计研究有限责任公司; 湖北 武汉 430010）
Keywords:
low temperature;  SBBR;  nitrogen and phosphorus removal;  community
摘要:
以SBBR高标准脱氮除磷系统为研究对象，考察了温度对该系统脱氮除磷效能及微生物种群的影响，提出了低温下SBBR系统高标准脱氮除磷的运行策略。结果表明，温度对系统的脱氮效能影响显著。当温度为10 ℃、COD负荷为0.82 kg/（m3·d）时，系统对NH4+-N、TN的平均去除率分别为72.4%、70.1%，与温度为24 ℃时相比，去除率分别降低26.4%和26.1%；通过调整低温（10 ℃）下SBBR系统的运行工况及进水方式，可以有效强化SBBR脱氮除磷效能，在COD负荷为0.41 kg/（m3·d）、运行工况为进水6 min—厌氧2 h—好氧9 h—缺氧3 h（二次进水）—好氧9 h—沉淀0.8 h—排水6 min条件下，对COD、NH4+-N、TN和PO43--P的去除率分别提高至95.3%、99.2%、95.9%、95.1%，出水水质达到《地表水环境质量标准》（GB 3838—2002）类Ⅳ类水标准。16S rRNA Amplicon高通量测序结果表明，温度对功能微生物种群结构的影响不显著，对脱氮除磷功能菌属的相对丰度影响显著。当温度由24 ℃降低至10 ℃时，硝化菌属Nitrospira、反硝化菌属Flavobacterium和Thermomonas、聚磷菌属Candidatus_Accumulibacter的相对丰度分别由1.47%、9.17%、6.27%、2.70%降至0.67%、2.66%、1.82%、0.30%；而异养硝化好氧反硝化菌属Pseudomonas、反硝化聚磷功能菌属Dechloromonas的相对丰度分别由0.07%、0.12%上升至1.05%、1.03%。
Abstract:
The effects of temperature on nitrogen and phosphorus removal efficiency and microbial community of a sequencing batch biofilm reactor (SBBR) under strict discharge standard were investigated, and the operational strategy for SBBR to achieve efficient nitrogen and phosphorus removal at low temperature was introduced. The temperature had a significant effect on the nitrogen removal efficiency of the system. When the temperature and COD loading rate were 10 ℃ and 0.82 kg/(m3·d), the average removal efficiencies of NH4+-N and TN were 72.4% and 70.1%, respectively, which decreased by 26.4% and 26.1% compared with those at 24 ℃. The removal efficiency of nitrogen and phosphorus was effectively enhanced by adjusting the operating condition and feeding mode of the SBBR at low temperature (10 ℃). When the operating conditions were as follows: COD loading rate of 0.41 kg/(m3·d) and cyclic time consisting of filling (6 min), anaerobic reaction (2 h), aerobic reaction (9 h), anoxic reaction (3 h, secondary filling), aerobic reaction (9 h), settling (0.8 h) and drainage (6 min), the removal efficiencies of COD, NH4+-N, TN and PO43--P increased to 95.3%, 99.2%, 95.9% and 95.1%, respectively,and the effluent quality met the class Ⅳ criteria specified in Environmental Quality Standards for Surface Water (GB 3838-2002). The 16S rRNA Amplicon high?throughput sequencing indicated that temperature had no significant effect on the microbial community composition, but had a significant effect on the relative abundance of denitrifying and phosphorous accumulating genera. When the temperature decreased from 24 ℃ to 10 ℃, the relative abundance of Nitrospira (nitrifying bacterium), Flavobacterium,Thermomonas (denitrifying bacteria) and Candidatus_Accumulibacter (phosphorous accumulating bacterium) decreased from 1.47%, 9.17%, 6.27% and 2.70% to 0.67%, 2.66%, 1.82% and 0.30%, respectively. In contrast, the relative abundance of Pseudomonas (heterotrophic nitrification and aerobic denitrification bacterium) and Dechloromonas (denitrifying phosphorus accumulating bacterium)increased from 0.07% and 0.12% to 1.05% and 1.03%, respectively.
更新日期/Last Update: 2022-05-01