LI Hui-li,HE Fu-rong,LIU Peng-cheng,et al.Optimization of Anaerobic Co-digestion Conditions of Straw and Sludge Enhanced by Exogenous Enzymes[J].China Water & Wastewater,2020,36(15):6-12.
外源酶强化秸秆污泥混合厌氧消化条件优化
- Title:
- Optimization of Anaerobic Co-digestion Conditions of Straw and Sludge Enhanced by Exogenous Enzymes
- Keywords:
- corn straw; municipal sludge; anaerobic digestion; cellulase; protease
- 摘要:
- 为研究不同外源酶对中温（35 ℃）混合厌氧消化过程的影响,以秸秆污泥混合物（质量比为1 ∶2）作为底物，在最优投加量下将蛋白酶与纤维素酶按1 ∶1、1 ∶2、2 ∶1、1 ∶3、3 ∶1等5组投加比设置实验，分析各组产气量、甲烷含量、发酵过程氨氮含量以及多糖、蛋白质、挥发性脂肪酸（VFAs）的含量和酶活的变化情况。结果表明,中性蛋白酶∶纤维素酶为1 ∶2的酶配比甲烷产量最大，为373.05 mL，TS和VS去除率分别达到10%和43.5%，且无VFAs积累，可以作为秸秆污泥混合厌氧消化最佳外源酶投加量。通过HPLC对多酶联合反应消化液进行测定，发现厌氧消化产物中高分子物质主要是生物聚合物和高分子质量腐殖酸。
- Abstract:
- Effects of exogenous enzymes on anaerobic co-digestion at medium temperature (35 ℃) were explored. In batch experiments, the mixture of straw and sludge (mass ratio=1 ∶2) was used as the substrate. Under the optimal dosage, five groups of protease and cellulase with the ratio of 1 ∶1, 1 ∶2, 2 ∶1, 1 ∶3 and 3 ∶1 were set. The gas production, methane content, ammonia nitrogen, polysaccharide, protein, volatile fatty acids (VFAs) and enzyme activity were analyzed. The maximum methane production (373.05 mL) with the TS removal efficiency of 10% and the VS removal efficiency of 43.5% was obtained when the ratio of neutral protease and cellulase was 1 ∶2, and there was no VFAs accumulation. Therefore, the enzyme ratio of 1 ∶2 could be used as the optimal dosage of exogenous enzymes for anaerobic codigestion of straw and sludge. The slurry of multi-enzyme combined reaction was determined by HPLC. It was found that the main macromolecule substances of anaerobic digestion products were biopolymer and high molecular weight humic acid.
相似文献/References:
[1]鲁涛,朱宝飞,石春梅,等.污泥碳化系统试运行及污泥炭回归土壤可行性分析[J].中国给水排水,2018,34(21):108.
LU Tao,ZHU Bao fei,SHI Chun mei,et al.Test Run of Sewage Sludge Carbonization System and Feasibility Analysis of Sludge Carbon Land Use[J].China Water & Wastewater,2018,34(15):108.
[2]孙建升,叶雅丽,郑兴灿,等.昆明市污水处理厂污泥处理工艺的研究与探讨[J].中国给水排水,2020,36(18):108.
SUN Jian-sheng,YE Ya-li,ZHENG Xing-can,et al.Research and Discussion on the Sludge Disposal Technology of Kunming WWTPs[J].China Water & Wastewater,2020,36(15):108.
[3]张会文,代晓炫,姜伟,等.市政污泥的水热反应减量化及水分赋存形态研究[J].中国给水排水,2021,37(7):96.
ZHANG Hui-wen,DAI Xiao-xuan,JIANG Wei,et al.Reduction and Water Forms of Municipal Sludge Treated by Hydrothermal Reaction[J].China Water & Wastewater,2021,37(15):96.
[4]吕开雷,杨淘,陈紫君,等.电解铝烟气余热低温干化处理市政污泥工程应用[J].中国给水排水,2021,37(12):120.
Lü Kai-lei,YANG Tao,CHEN Zi-jun,et al.Application of Low Temperature Drying in Municipal Sludge Treatment with Waste Heat of Electrolytic Aluminum Flue Gas[J].China Water & Wastewater,2021,37(15):120.
[5]李义烁,梁远,颜莹莹,等.餐厨垃圾/市政污泥/城市粪便联合厌氧消化沼液处理设计[J].中国给水排水,2021,37(14):56.
LI Yi-shuo,LIANG Yuan,YAN Ying-ying,et al.Design of Treatment Process of Biogas Slurry from Anaerobic Co-digestion of Kitchen Waste/Municipal Sludge/Urban Excrement[J].China Water & Wastewater,2021,37(15):56.
[6]李彪,周欣,高波,等.顺义区污泥干化焚烧处理工程工艺设计[J].中国给水排水,2021,37(14):63.
LI Biao,ZHOU Xin,GAO Bo,et al.Design of Sludge Drying Incineration Treatment Process in Shunyi District[J].China Water & Wastewater,2021,37(15):63.
[7]东东,赵珊,郭学彬,等.典型污泥高级厌氧消化工艺中恶臭物质的分布特征[J].中国给水排水,2021,37(21):7.
DONG Dong,ZHAO Shan,GUO Xue-bin,et al.Distribution Characteristics of Odorants in Typical Sludge Advanced Anaerobic Digestion Process[J].China Water & Wastewater,2021,37(15):7.
[8]程文,蒋岚岚,耿震,等.太原市城市污水厂污泥资源化利用工程设计[J].中国给水排水,2022,38(24):72.
CHENGWen,JIANGLan-lan,GENGZhen,et al.Engineering Design of Sludge Resource Utilization in Taiyuan Municipal Wastewater Treatment Plant[J].China Water & Wastewater,2022,38(15):72.
[9]李思敏,李思雨,唐锋兵,等.通风速率对市政污泥好氧堆肥氮素转化的影响[J].中国给水排水,2023,39(5):121.
LISi-min,LISi-yu,TANGFeng-bing,et al.Effect of Ventilation Rate on Nitrogen Transformation of Municipal Sludge in Aerobic Composting[J].China Water & Wastewater,2023,39(15):121.
[10]刘欣欣,庞敏,李会东,等.污泥基磁性吸附剂制备及其去除废水中铜的研究[J].中国给水排水,2023,39(21):99.
LIUXin-xin,PANGMin,LIHui-dong,et al.Preparation of Sludge-based Magnetic Adsorbent and Its Removal of Copper from Wastewater[J].China Water & Wastewater,2023,39(15):99.