[1]刘国臣,赵志伟,梁志杰,等.多点进水与弱电压刺激提高斑马鱼报警的精确度[J].中国给水排水,2021,37(7):42-48.
　LIU Guo-chen,ZHAO Zhi-wei,LIANG Zhi-jie,et al.Improving the Accuracy of Zebrafish Alarm through Multi-point Water Intake and Weak Voltage Stimulation[J].China Water & Wastewater,2021,37(7):42-48.
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多点进水与弱电压刺激提高斑马鱼报警的精确度

中国给水排水[ISSN:1000-4062/CN:12-1073/TU] 卷: 第37卷 期数: 2021年第7期 页码: 42-48 栏目: 出版日期: 2021-04-01
Title:
Improving the Accuracy of Zebrafish Alarm through Multi-point Water Intake and Weak Voltage Stimulation
作者:
刘国臣，赵志伟，梁志杰，崔福义，阳春，李秀麟，陈瑞
(重庆大学环境与生态学院三峡库区生态环境教育部重点实验室，重庆400044)
Author(s):
LIU Guo-chen,ZHAO Zhi-wei,LIANG Zhi-jie,CUI Fu-yi,YANG Chun,LI Xiu-lin,CHEN Rui
(Key Laboratory of Three Gorges Reservoir Region’s Eco-Environment , School of Environment and Ecology, Chongqing University, Chongqing 400044, China)
关键词:
生物监测; 水质预警; 斑马鱼; 重金属离子
Keywords:
biological monitoring; water quality warning; zebrafish; heavy metal ions
摘要:
为了提高斑马鱼在实际水质毒性监测应用中报警的准确性和稳定性，通过改进实验装置，在监测养殖箱左右3 cm处各安置一块上面带有直径为1 cm圆孔的铝板，改变污染物的扩散方式，同时在铝板左右加上变频电压而形成稳定电场，刺激中毒反应前后的斑马鱼。结果表明：多点进水方式下污染物可以更快更均匀地扩散到养殖箱中并与斑马鱼接触，提前约10 min报警，缩短了报警时间，提高了报警的灵敏度。可以选择电压为10 V，间隔10 min刺激一次毒物暴露后的斑马鱼，刺激时长为1 min作为检验报警发生的真假，提高报警的准确度。在毒物暴露后的0.5~1.5 h之间设置间歇性的10 V电刺激，若斑马鱼的速度、转次变化行为明显低于空白，则认为报警为真。
Abstract:
In order to improve the accuracy and stability of zebrafish in actual water toxicity monitoring applications, an improved experiment was carried out. In the study, an aluminum plate with a 1 cm diameter hole was placed at 3 cm left and right of the monitoring breeding box to change the diffusion method of pollutants. At the same time, the frequency conversion voltage was added to the left and right of the aluminum plate to form a stable electric field and stimulate the zebrafish before and after the poisoning reaction. The results showed that in the multi-point water intake mode, the pollutants could diffuse into the breeding box more quickly and evenly, and contact with the zebrafish. The alarm was about 10 minutes in advance and improved the sensitivity of the alarm. A voltage of 10 V could be selected to stimulate the zebrafish after the poison exposure every 10 min, and the stimulation time was 1 min to check whether the alarm was true or false, and improved the accuracy of the alarm. Intermittent 10 V electrical stimulation was set between 0.5-1.5 h after exposure to the poison. If the speed and turnover behavior of the zebrafish was significantly lower than the blank, the alarm was considered true.
更新日期/Last Update: 2021-04-01