[1]曾小康,孙士权,高阳,等.餐厨垃圾水解液发酵产油脂特性及动力学研究[J].中国给水排水,2021,37(15):83-88.
　ZENG Xiao-kang,SUN Shi-quan,GAO Yang,et al.Characteristics and Kinetics of Oil Production from Kitchen Waste Hydrolysate Fermentation[J].China Water & Wastewater,2021,37(15):83-88.
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餐厨垃圾水解液发酵产油脂特性及动力学研究

中国给水排水[ISSN:1000-4062/CN:12-1073/TU] 卷: 第37卷 期数: 2021年第15期 页码: 83-88 栏目: 出版日期: 2021-08-01
Title:
Characteristics and Kinetics of Oil Production from Kitchen Waste Hydrolysate Fermentation
作者:
曾小康1,2,3，孙士权1,3，高阳1,胡文涛1，孙欣悦1，蒋昌波1，吴方同1，万俊力1，周璐1
(1.长沙理工大学水利工程学院，湖南长沙410114；2.中国电建集团中南勘测设计研究院有限公司，湖南长沙410014；3.湖南省环境保护河湖污染控制工程技术中心，湖南长沙410114)
Author(s):
ZENG Xiao-kang1,2,3,SUN Shi-quan1,3,GAO Yang1，HU Wen-tao1，SUN Xin-yue1,JIANG Chang-bo1,WU Fang-tong1,WAN Jun-li1,ZHOU Lu1
(1. School of Hydraulic Engineering, Changsha University of Science and Technology， Changsha 410114， China； 2. PowerChina Zhongnan Engineering Corporation Limited, Changsha 410014， China; 3. Engineering and Technical Center of Hunan Provincial Environmental Protection for River-Lake Dredging Pollution Control, Changsha 410114， China)
关键词:
餐厨垃圾水解液; 酿酒酵母; 微生物油脂; 发酵; 动力学
Keywords:
kitchen waste hydrolysate; Saccharomyces cerevisiae; microbial oils; fermentation; kinetics
摘要:
为探究餐厨垃圾水解液发酵产油脂的动力学特征和影响因素，以Saccharomyces cerevisiae As2.516（S. cerevisiae As2.516）为供试菌株，在初始餐厨垃圾水解液加入量为90%、搅拌速率为180 r/min、通气量为2.5 L/min、发酵周期为10 d的条件下，进行1 L发酵罐发酵实验。实验结果表明，S. cerevisiae As2.516的最优初始培养条件如下：pH值为6、温度为30 ℃、生物接种量为10%；在此实验条件下，发酵第7天油脂产量最高达4.6 g/L，此时生物量为13 g/L，油脂产率为36.1%；发酵产生的脂肪酸主要由C16和C18脂肪酸组成。餐厨垃圾水解液中的无机盐钠（Na+）、钾（K+）、铁（Fe3+）、镁（Mg2+）、锰（Mn2+）含量丰富，除需添加微量硫酸铜（CuSO4·5H2O，0.1 mg/L）外，无需额外添加其他无机盐。同时基于Logistic方程和Luedeking-Piret等方程建立了S. cerevisiae As2.516菌体生长、产物生成、底物还原糖消耗3个发酵动力学模型，较好地描述了餐厨垃圾水解液培养菌体产油脂发酵过程中菌体生长、油脂合成以及底物还原糖消耗的过程。
Abstract:
To investigate the kinetic characteristics and influencing factors of oil production from kitchen waste hydrolysate fermentation, a fermentation experiment was carried out in a 1 L fermenter, in which Saccharomyces cerevisiae As2.516 (S. cerevisiae As2.516) was used as a test strain the dosage of initial kitchen waste hydrolysate was 90%, agitation speed was 180 r/min, aeration rate was 2.5 L/min and fermentation cycle was 10 days. The initial optimal culture conditions for S. cerevisiae As2.516 were as follows: pH of 6, temperature of 30 ℃ and inoculation percentage of 10%. Based on the optimum culture condition, the highest oil yield was 4.6 g/L on the 7th day of fermentation, the biomass was 13 g/L and the oil yield rate was 36.1%. Fatty acids produced by fermentation were mainly composed of C16 and C18 fatty acids. Contents of sodium (Na+), potassium (K+), iron (Fe3+), magnesium (Mg2+) and manganese (Mn2+) in the kitchen waste hydrolysate were abundant, and there was no need to add other inorganic salts except for adding trace copper sulfate (CuSO4·5H2O, 0.1 mg/L). In addition, three fermentation kinetics models of S. cerevisiae As2.516 growth, production formation and substrate (reducing sugar) depletion were established based on Logistic and Luedeking-Piret equations, and the process of cell growth, oil synthesis and substrate (reducing sugar) consumption could be well described by these models.
更新日期/Last Update: 2021-08-01