[1]刘小英,陈远,曾翱,等.AGS系统丝状菌过度生长前后微生物群落结构对比[J].中国给水排水,2021,37(19):22-27.
　LIU Xiao-ying,CHEN Yuan,ZENG Ao,et al.Comparison of Microbial Community Composition before and after Overgrowth of Filamentous Bacteria in AGS System[J].China Water & Wastewater,2021,37(19):22-27.
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AGS系统丝状菌过度生长前后微生物群落结构对比

中国给水排水[ISSN:1000-4062/CN:12-1073/TU] 卷: 第37卷 期数: 2021年第19期 页码: 22-27 栏目: 出版日期: 2021-10-01
Title:
Comparison of Microbial Community Composition before and after Overgrowth of Filamentous Bacteria in AGS System
作者:
刘小英1，陈远1,2，曾翱1,3，秦诗友4,李仁杰1
(1.武汉理工大学土木工程与建筑学院，湖北武汉430070；2.北京市市政工程设计研究总院有限公司，北京100082;3.中国市政工程中南设计研究总院有限公司，湖北武汉430014；4.武汉市政工程设计研究院有限责任公司，湖北武汉430011)
Author(s):
LIU Xiao-ying1,CHEN Yuan1,2,ZENG Ao1,3,QIN Shi-you4,LI Ren-jie1
(1. School of Civil Engineering and Architecture, Wuhan University of Technology, Wuhan 430070, China; 2. Beijing General Municipal Engineering Design & Research Institute Co. Ltd., Beijing 100082, China; 3. Central and Southern China Municipal Engineering Design & Research Institute Co. Ltd., Wuhan 430014, China; 4. Wuhan Municipal Engineering Design & Research Institute Co. Ltd., Wuhan 430011, China)
关键词:
颗粒污泥; 丝状菌; 过度生长; 微生物群落结构; 高通量测序
Keywords:
granular sludge; filamentous bacteria; overgrowth; microbial community composition; high-throughput sequencing
摘要:
微生物群落结构是影响颗粒污泥反应器性能的主要因素，而丝状菌的生长状态对颗粒污泥系统性能及稳定性具有重要作用。基于此，借助高通量测序手段对比分析了丝状菌正常生长和过度生长时颗粒污泥中微生物群落结构的变化。研究表明，丝状菌过度生长后，微生物多样性显著下降，两种情况下颗粒污泥在属水平上仅有4种相同的菌属。当丝状菌正常生长时，优势菌属为Candidatus_Saccharimonas、Candidatus_Accumulibacter、Thauera、Lewinella;当丝状菌过度生长时，优势菌属为Hydrogenophaga、Gemmobacter、Opitutus、Meganema、Pseudoxanthomonas，微生物群落结构变化显著。随着丝状菌的过度生长，具有硝化和除磷功能的菌属相对丰度有所下降，而具有反硝化功能的菌属相对丰度由31.29%显著增加到52.99%，同时丝状菌的相对丰度也由7.99%增加到19.46%，这一变化与反应器性能和污泥特性变化相一致。
Abstract:
Microbial community composition is the main factor affecting the performance of granular sludge reactor, and the growth of filamentous bacteria plays an important role in the performance and stability of the granular sludge. Therefore, the variations in microbial community compositions of granular sludge with normal growth and overgrowth of filamentous bacteria were compared and analyzed by high-throughput sequencing. After the overgrowth of filamentous bacteria, the microbial diversity decreased significantly, and there were only 4 identical species in the two kinds of granular sludge at the genus level. When the filamentous bacteria grew normally, the dominant genera were Candidatus_Saccharimonas, Candidatus_Accumulibacter, Thauera and Lewinella, and the dominant genera were Hydrogenophaga, Gemmobacter, Opitutus, Meganema and Pseudoxanthomonas when the filamentous bacteria overgrew, indicating that the microbial community composition changed significantly. With the overgrowth of the filamentous bacteria, the relative abundance of genera with nitrification and phosphorus removal ability decreased to some extent. However, the relative abundance of genera with denitrifying ability increased significantly from 31.29% to 52.99%, and the relative abundance of filamentous bacteria increased from 7.99% to 19.46%, which was consistent with the change of the reactor performance and the sludge characteristics.

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更新日期/Last Update: 2021-10-01