YU Li-fang,MO Peng-cheng,YANG Xiu-ling,et al.Strategy for Nitrite Oxidizing Bacteria Adapting to Low Temperature during Long-term Operation[J].China Water & Wastewater,2021,37(23):68-72.
长期运行条件下亚硝酸盐氧化菌的低温适应策略
- Title:
- Strategy for Nitrite Oxidizing Bacteria Adapting to Low Temperature during Long-term Operation
- 关键词:
- 低温; 亚硝酸盐氧化菌; Nitrotoga; Nitrospira
- 摘要:
- 在20、15和10 ℃条件下长期运行SBR，分析温度对反应器性能、硝化动力学和硝化菌群落结构等的影响。实验结果表明，随着温度的降低，比氨氧化速率（SAUR）逐渐降低，比亚硝酸盐氧化速率（SNUR）没有显著变化，氨氧化菌(AOB)和亚硝酸盐氧化菌(NOB)的长期温度修正系数分别为1.078和1.007 8，NOB的低温适应性强于AOB；定量PCR结果显示，AOB丰度随着温度的降低而下降，而NOB丰度没有明显变化；但NOB中嗜冷菌Nitrotoga随着温度的降低而逐渐增加，并取代Nitrospira成为NOB的优势菌属，从而在一定程度上缓解因Nitrospira减少而引起的SNUR降低。因此，NOB群落结构由Nitrospira向Nitrotoga迁移是其适应低温环境的关键。
- Abstract:
- A sequencing batch reactor(SBR) was operated continuously at 20 ℃,15 ℃ and 10 ℃ in a long-running process, and effects of temperature on reactor performance, nitrification kinetics and nitrifying bacterial community structure were analyzed. With the decrease of temperature, specific ammonia uptake rate (SAUR) decreased gradually, but specific nitrite uptake rate(SNUR) did not change significantly.Arrhenius coefficients of ammonia oxidizing bacteria(AOB) and nitrite oxidizing bacteria (NOB) during long-term operation were 1.078 and 1.007 8, respectively,indicating that NOB was more adaptable to low temperature than AOB.Quantitative PCR indicated that the abundance of AOB decreased with decreasing temperatures, while the abundance of NOB did not change significantly. Nitrotoga, a psychrophilic bacterium in NOB, increased gradually with the decrease of temperature and replaced Nitrospira to become the dominant bacterium in NOB, thus reducing the SNUR decrease caused by the decrease of Nitrospira to a certain extent. Therefore, the migration of NOB community structure from Nitrospira to Nitrotoga was the key to its adaptation to low temperature environment.
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