[1]冉治霖,毛竹,相会强,等.UV-LED/氯工艺灭活枯草芽孢杆菌机理研究[J].中国给水排水,2022,38(11):43-48.
　RANZhi-lin,MAOZhu,XIANGHui-qiang,et al.Mechanism of Bacillus subtilis Inactivated by Ultraviolet Light-emitting Diode and Chlorine[J].China Water & Wastewater,2022,38(11):43-48.
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UV-LED/氯工艺灭活枯草芽孢杆菌机理研究

中国给水排水[ISSN:1000-4062/CN:12-1073/TU] 卷: 第38卷 期数: 2022年第11期 页码: 43-48 栏目: 出版日期: 2022-06-01
Title:
Mechanism of Bacillus subtilis Inactivated by Ultraviolet Light-emitting Diode and Chlorine
作者:
冉治霖1,2,3， 毛竹3,4， 相会强1,2， 李绍峰3， 聂锦旭4， 王志红4
（1.深圳信息职业技术学院 创新教育研究院，广东 深圳 518172；2.深圳信息职业技术学院 交通与环境学院，广东 深圳 518172；3.深圳职业技术学院 深圳市工业节水及城市污水资源化技术重点实验室，广东 深圳 518055；4.广东工业大学 土木与交通工程学院，广东 广州 510006）
Author(s):
RAN　Zhi-lin1，2，3， MAO　Zhu3，4， XIANG　Hui-qiang1，2， LI　Shao-feng3， NIE　Jin-xu4， WANG　Zhi-hong4
（1. Institute of Innovational Education Research， Shenzhen Institute of Information Technology，Shenzhen 518172， China； 2. School of Traffic and Environment， Shenzhen Institute of Information Technology， Shenzhen 518172， China； 3. Shenzhen Key Laboratory of Industrial Water Saving and Municipal Sewage Reclamation Technology， Shenzhen Polytechnic， Shenzhen 518055， China；4. School of Civil and Transportation Engineering， Guangdong University of Technology， Guangzhou 510006， China）
关键词:
紫外发光二极管;  氯;  枯草芽孢杆菌;  灭活机理
Keywords:
ultraviolet light-emitting diode;  chlorine;  Bacillus subtilis;  inactivation mechanism
摘要:
采用紫外发光二极管（UV-LED）联合次氯酸钠灭活枯草芽孢杆菌，并分析了单独UV-LED和单独氯的灭活效果。结果发现，UV-LED/氯体系中存在着协同作用，灭活效果远好于单独UV-LED和单独氯。蛋白质溶出、暗修复和细胞表面结构情况分析表明，产生的自由基破坏了细胞外部结构，致使次氯酸分子和次氯酸根离子进入细胞内，同时被破坏的细胞更易于UV穿透，二者共同作用造成了DNA受损，最终导致其失活。此外，评估了UV-LED、羟基自由基、氯、氯自由基和次氯基在UV-LED/氯灭活过程中的贡献，发现UV-LED和羟基自由基贡献较大。
Abstract:
Bacillus subtilis was inactivated by ultraviolet light-emitting diode (UV-LED) combined with sodium hypochlorite, and the inactivation performance of UV-LED and chlorine alone was analyzed. Synergistic effect existed in the UV-LED/chlorine system, and the inactivation performance was much better than that of UV-LED and chlorine alone. Protein dissolution, dark repair and cell surface structure showed that the produced free radicals damaged the outer structure of the cell, leading to the entry of hypochlorous acid molecules and hypochlorite ions into the cells, while the damaged cells were more susceptible to UV penetration. The combination of the two actions caused DNA damage and ultimately inactivated the bacteria. In addition, the contributions of UV-LED, hydroxyl radical, chlorine, chlorine radical and hypochlorous acid to UV-LED/chlorine inactivation were evaluated, and it was found that the contribution of UV-LED and hydroxyl radical was larger.
更新日期/Last Update: 2022-06-01