MENGXiao-jun,ZUDe-biao,ZHANGYu-xiu,et al.Denitrification Performance of Heterotrophic Nitrification-Aerobic Denitrification Bacteria HNAD4[J].China Water & Wastewater,2022,38(15):29-36.
异养硝化-好氧反硝化菌HNAD4的脱氮性能分析
- Title:
- Denitrification Performance of Heterotrophic Nitrification-Aerobic Denitrification Bacteria HNAD4
- 关键词:
- 焦化废水; 异养硝化-好氧反硝化; 同步硝化反硝化; 生物脱氮
- Keywords:
- coking wastewater; heterotrophic nitrification-aerobic denitrification; simultaneous nitrification and denitrification; biological denitrification
- 摘要:
- 异养硝化-好氧反硝化为焦化废水处理革新提供了新思路。从某焦化废水处理厂活性污泥中分离获得一株高效的异养硝化-好氧反硝化菌株HNAD4，经16S rRNA鉴定为Pseudomonas sp.。分析了菌株HNAD4的硝化、反硝化以及同步硝化反硝化（SND）性能，研究了菌株对实际焦化废水的处理能力。结果表明，当菌株以柠檬酸钠为碳源、C/N为15、温度为35 ℃、pH为7.0时，硝化性能最优；以NO3--N和NO2--N为混合氮源时，两种氮源可同步去除，但反硝化优先利用NO3--N；以NH4+-N、NO3--N和NO2--N为混合氮源时，SND过程中利用氮源的优先顺序依次为NH4+-N、NO3--N和NO2--N；当处理实际焦化废水时，与对照组相比，投加菌株的实验组可使A/O1/O2工艺中O1池的NH4+-N去除率提高46.45%、TN去除率提高33.14%。
- Abstract:
- Heterotrophic nitrification-aerobic denitrification provides a new idea for the innovation of coking wastewater treatment. An efficient heterotrophic nitrification-aerobic denitrification strain HNAD4 was isolated from the activated sludge of coking wastewater treatment plant and identified as Pseudomonas sp. by 16S rRNA. The nitrification, denitrification and simultaneous nitrification and denitrification (SND) characteristics of strain HNAD4 were analyzed, and the ability of the strain for actual coking wastewater treatment was investigated. The best nitrification performance was obtained when the following operating conditions were adopted: sodium citrate as carbon source, C/N ratio of 15, temperature of 35 ℃ and pH of 7.0. When NO3--N and NO2--N were used as mixed nitrogen sources, the two nitrogen sources could be simultaneously removed, but NO3--N was preferentially used in denitrification. When NH4+-N, NO2--N and NO3--N were used as mixed nitrogen sources, the priority of nitrogen sources utilized in SND process was NH4+-N, NO3--N and NO2--N. When the actual coking wastewater was treated, the removal efficiencies of NH4+-N and TN in O1 tank of A/O1/O2 process increased by 46.45% and 33.14% in the experimental group compared with those in the control group.
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