ZHANGBing,WULian,HUANGShu-chang,et al.Effect of Different Operational Modes and C/N Ratios on Enhanced Partial Nitrification and Denitrification of AGS[J].China Water & Wastewater,2023,39(1):9-18.
运行模式及C/N对强化AGS短程硝化反硝化的影响
- Title:
- Effect of Different Operational Modes and C/N Ratios on Enhanced Partial Nitrification and Denitrification of AGS
- Keywords:
- aerobic granular sludge; partial nitrification and denitrification; operational mode; C/N ratio; microbial community structure
- 摘要:
- 为了强化好氧颗粒污泥（AGS）的短程硝化反硝化，对序批式反应器（SBR）的运行模式和进水C/N进行了优化。结果表明：R2（厌氧/好氧+厌氧/好氧）中的AGS培养至第56天时，丝状菌膨胀严重导致反应器暂停运行；而R1（厌氧/好氧）的污泥性能及污染物去除效果均优于R2。R1在C/N为6、7.5时短暂出现短程硝化反硝化现象，继续提高C/N至10，NO2--N出现明显积累，亚硝态氮积累率（NAR）达到100%，成功实现了强化AGS短程硝化反硝化脱氮的目的，系统对COD、TIN、TP的去除率分别达到95%、75%、70%以上。高通量测序结果表明，颗粒化后R1系统内的微生物群落结构与接种污泥差别较大。在属水平上，接种污泥中Candidatus_Competibacter（0.28%）、Flavobacterium（0.03%）的相对丰度较低，R1在实现污泥颗粒化后，多种反硝化细菌如Candidatus_Competibacter（13.53%）、Thauera（3.25%）等得到了明显富集，此外，Flavobacterium（13.66%）、Comamonas（2.23%）是优势积累的氨氧化细菌（AOB），推测这些细菌相互合作，共同实现了AGS系统的高效短程硝化反硝化脱氮。
- Abstract:
- In order to enhance the partial nitrification and denitrification of aerobic granular sludge (AGS), the operational modes, including anaerobic/aerobic (R1) and anaerobic/aerobic+anaerobic/aerobic (R2)，and the influent C/N ratios (6, 7.5, 10) of the sequencing batch reactor (SBR) were optimized. The results showed that when the AGS in R2 was cultured to day 56, the filamentous bacteria seriously bulked, leading to the suspension of the reactor. However, granules in R1 had better settling performance and pollutant removal efficiency compared to R2. When C/N ratio of R1 was 6 and 7.5, the partial nitrification and denitrification occurred briefly. When the C/N ratio was increased to 10, NO2--N significantly accumulated, and NAR achieved 100%, the purpose of enhancing AGS partial nitrification and denitrification was successfully realized in R1, and the removal rates of COD, TIN and TP were above 95%,75% and 70%, respectively. The results of high?throughput sequencing showed that the microbial community structure in R1 system after granulation was significantly different from that of inoculated sludge. At the genus level, the genera Candidatus_Competibacter (0.28%) and Flavobacterium (0.03%) in the inoculated sludge had relative lower abundance. After the implementation of sludge granulation in R1, a variety of denitrifying bacteria such as Candidatus_Competibacter (13.53%) and Thauera (3.25%) were noticeably enriched. In addition, the genera Flavobacterium (13.66%) and Comamonas (2.23%) were the dominant ammonia oxidizing bacteria (AOB). It was speculated that these bacteria cooperated with each other to achieve efficient partial nitrification and denitrification in AGS system.
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