WANGChao-chao,ZHANGXiao-nong,LIJia-jia,et al.Effect of Carbon Sources on Performance of Partial Denitrification and Anaerobic Ammonia Oxidation Process[J].China Water & Wastewater,2023,39(3):15-24.
碳源对短程反硝化-厌氧氨氧化工艺效能的影响
- Title:
- Effect of Carbon Sources on Performance of Partial Denitrification and Anaerobic Ammonia Oxidation Process
- 关键词:
- 厌氧氨氧化; 短程反硝化; 碳源; COD/NO3--N; 生物膜-活性污泥
- Keywords:
- anaerobic ammonia oxidation (Anammox); partial denitrification; carbon source; COD to NO3--N ratio; biofilm-activated sludge
- 摘要:
- 为探究大分子有机碳源对短程反硝化-厌氧氨氧化（PD/A）系统脱氮效能的影响，通过在厌氧氨氧化（Anammox）连续流反应器中投加葡萄糖和新型组合填料快速启动生物膜-活性污泥PD/A工艺，研究了不同碳源条件（单一碳源和混合碳源）和不同COD/NO3--N下该工艺的脱氮性能。结果表明，以葡萄糖为唯一碳源，控制COD/NO3--N为4.5，在第90天成功启动PD/A系统，TN去除率达到93.89%，出水TN为6.17 mg/L，此时Anammox过程对去除TN的贡献率高达95.83%，反硝化过程的贡献率仅为4.17%；而由于短程反硝化菌对淀粉和葡萄糖混合碳源的利用率下降，在混合碳源驱动下PD/A系统的TN去除率降至90.53%（COD/NO3--N=5），出水TN为9.19 mg/L，且反硝化过程对去除TN的贡献率升至8.48%。微生物高通量测序结果表明，在两种碳源条件下，变形菌门均为绝对优势菌门，其相对丰度均大于50%；Candidatus_Brocadia和Thauera作为PD/A系统的优势菌属，在氮素协同去除方面发挥了重要作用，其在混合碳源条件下的相对丰度分别为2.97%和6.52%，略低于单一碳源条件下的3.41%和6.94%；厌氧水解酸化菌属的相对丰度较低是混合碳源利用率下降的主要原因。此外，提出了一种新型生物膜-活性污泥Anammox主流脱氮工程应用方案。
- Abstract:
- To investigate the effect of macromolecular organic carbon sources on nitrogen removal efficiency of partial denitrification and anaerobic ammonia oxidation (PD/A) system, the biofilm-activated sludge PD/A process was rapidly started by adding glucose and new combined packing media in the Anammox continuous flow reactor, and the effects of different carbon source conditions (single carbon source and mixed carbon sources) and different COD to NO3--N ratios (COD/NO3--N) on nitrogen removal performance of the process were explored. When glucose was the sole carbon source and COD/NO3--N was 4.5, the PD/A system was successfully started on the 90th day. The removal rate of TN reached 93.89%, the TN in the effluent was 6.17 mg/L, the contribution rate of Anammox to TN removal was 95.83%, and that of denitrification to TN removal was only 4.17%. Due to the decreased utilization rate of starch and glucose mixed carbon sources by PD bacteria, the TN removal rate of PD/A system decreased to 90.53% (COD/NO3--N of 5), the TN in the effluent was 9.19 mg/L, and the contribution rate of denitrification to TN removal increased to 8.48%. The microbial high-throughput sequencing results showed that Proteobacteria was always the absolutely dominant phylum under the two carbon source conditions, and the relative abundance was always greater than 50%. Candidatus_Brocadia and Thauera, as the dominant genera in the PD/A system, played an important role in the collaborative removal of nitrogen. The relative abundance of Candidatus Brocadia and Thauera under mixed carbon sources was 2.97% and 6.52% respectively, which was slightly lower than those under single carbon source (3.41% and 6.94%). The low relative abundance of anaerobic hydrolytic acidification bacteria was the main reason for the decreased utilization of mixed carbon sources. Therefore, the paper proposed a novel biofilm-activated sludge Anammox mainstream nitrogen removal scheme for engineering application.
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