[1]付正伟,朱峰,霍宗利,等.HPLC-MS/MS测定水中52种药品及个人护理品[J].中国给水排水,2023,39(4):127-138.
　FUZheng-wei,ZHUFeng,HUOZong-li,et al.Determination of 52 Pharmaceuticals and Personal Care Products in Water by HPLC-MS/MS[J].China Water & Wastewater,2023,39(4):127-138.
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HPLC-MS/MS测定水中52种药品及个人护理品

中国给水排水[ISSN:1000-4062/CN:12-1073/TU] 卷: 第39卷 期数: 2023年第4期 页码: 127-138 栏目: 出版日期: 2023-02-17
Title:
Determination of 52 Pharmaceuticals and Personal Care Products in Water by HPLC-MS/MS
作者:
付正伟1， 朱峰2， 霍宗利2， 杨红1， 张昊2
（1.东南大学 公共卫生学院，江苏 南京 210009；2.江苏省疾病预防控制中心，江苏 南京 210009）
Author(s):
FU　Zheng-wei1， ZHU　Feng2， HUO　Zong-li2， YANG　Hong1， ZHANG　Hao2
（1. School of Public Health， Southeast University， Nanjing 210009， China； 2. Jiangsu Provincial Center for Disease Control and Prevention， Nanjing 210009， China）
关键词:
药品及个人护理品;  高效液相色谱-串联质谱;  前处理
Keywords:
pharmaceuticals and personal care products;  HPLC-MS/MS;  pretreatment
摘要:
探索了不同样品前处理方法对高效液相色谱-串联质谱法（HPLC-MS/MS）测定水中52种药品及个人护理品（PPCPs）的影响，以提高测定方法的准确性和稳定性。研究利用HPLC-MS/MS法测定水样经不同前处理（不同材质滴管移取、避光与否、保存时间、离心、不同滤膜过滤）后PPCPs的浓度差异，以优化样品前处理条件。结果表明，经聚乙烯塑料滴管吸取、放置8 h避光与不避光造成水中PPCPs的最大损失率分别达36.4%、51.7%、60.2%。经离心或三种不同材质（PTFE、NL66、MCE）滤膜过滤后，52种PPCPs的回收率为0.0% ~114.2%。自来水基质加标实验结果显示，除5种大环内酯类抗生素和维吉尼霉素S1外，其余46种PPCPs经各自最优滤膜过滤后回收率均不小于73.3%。由此可见，水中部分PPCPs易被吸附、发生自身降解或光解，离心、过滤等手段会造成低浓度PPCPs的检测结果被低估。因此，实际检测中应将样品避光放置，采样后8 h内完成测定，避免使用聚乙烯滴管、聚丙烯离心管等塑料制品，并明确所使用的滤膜不会吸附待测PPCPs。
Abstract:
The effects of different sample pretreatment methods on high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) for the determination of 52 kinds of pharmaceutical and personal care products (PPCPs) in water were investigated to improve the accuracy and stability of the determination method. HPLC-MS/MS was used to determine the concentration difference of PPCPs in water samples after different pretreatment methods (transferring by different materials of dropper, whether to avoid light, storage time, centrifugation and filtration by different filter membranes), and then the pretreatment conditions of samples were optimized. The maximum loss ratio of PPCPs in water caused by polyethylene plastic dropper transferring, avoiding light and without avoiding light placing for 8 h was 36.4%, 51.7% and 60.2%, respectively. After centrifugation or filtration with three different materials (PTFE, NL66 and MCE), the recovery rates of 52 PPCPs were 0.0% to 114.2%. The result of the the tap water spiked experiment revealed that the recovery rates of 46 PPCPs, except five macrolides antibiotics and Virginiamycin S1, were all ≥73.3% after the filtration with the corresponding best filter membranes. The results indicated that some PPCPs in water were easy to be adsorbed, self-degraded or photodegraded, and the detection results of low concentration PPCPs were underestimated by means of centrifugation and filtration. Therefore, the samples should be placed avoiding light before determination, and the analysis should be completed within 8 h after sampling. In addition, the use of plastic products such as polyethylene dropper and polypropylene centrifuge tube should be avoided, and make sure that the filter membrane will not absorb the PPCPs to be tested.
更新日期/Last Update: 2023-02-17