[1]刘婷婷,程莉蓉,谢恩,等.磷源对蛋白核小球藻生长和产油性能的影响[J].中国给水排水,2023,39(7):81-88.
　LIUTing-ting,CHENG Li-rong,XIEEn,et al.Effects of Different Kinds of Phosphorus Source on Growth and Lipid Production of Chlorella pyrenoidosa[J].China Water & Wastewater,2023,39(7):81-88.
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磷源对蛋白核小球藻生长和产油性能的影响

中国给水排水[ISSN:1000-4062/CN:12-1073/TU] 卷: 第39卷 期数: 2023年第7期 页码: 81-88 栏目: 出版日期: 2023-04-01
Title:
Effects of Different Kinds of Phosphorus Source on Growth and Lipid Production of Chlorella pyrenoidosa
作者:
刘婷婷1， 程莉蓉1， 谢恩2， 郑蕾1， 丁爱中1， 黄绵松3,4
（1.北京师范大学 水科学研究院，北京 100875；2.中国农业大学 水利与土木工程学院，北京 100083；3.北京首创股份有限公司，北京 100000；4.宁夏首创海绵城市建设发展有限公司，宁夏 固原 756000）
Author(s):
LIU　Ting-ting1， CHENG Li-rong1， XIE　En2， ZHENG　Lei1， DING　Ai-zhong1， HUANG　Mian-song3，4
（1. College of Water Science， Beijing Normal University， Beijing 100875， China； 2. College of Water Resources and Civil Engineering， China Agricultural University， Beijing 100083， China； 3. Beijing Capital Co. Ltd.， Beijing 100000， China； 4. Ningxia Capital Sponge City Construction & Development Co. Ltd.， Guyuan 756000， China）
关键词:
蛋白核小球藻;  磷源;  产油;  共培养;  中性脂
Keywords:
Chlorella pyrenoidesa;  phosphorous source;  lipid production;  co-culture system;  neutral lipid
摘要:
微藻产油因其清洁无毒的特点被广泛关注，然而培养成本高、产油效率低成为其大量生产的瓶颈。为解决这一难题，探究了典型无机磷、常见有机磷、核苷磷酸、环形核苷磷酸四类磷源对单藻培养及菌藻共培养体系中蛋白核小球藻生长及产油情况的影响，用紫外分析和尼罗红染色法检测不同培养体系中藻细胞生长数目、中性脂产量及磷与碱性磷酸酶的变化情况。结果显示，两个体系对磷的利用情况无显著差异；纯藻培养条件下藻细胞数目（4.94×108 个/10 μL）高于菌藻共培养体系的（3.38×108 个/10 μL），而菌藻共培养体系的产油情况较好。以磷酸盐、三偏磷酸盐、磷酸三乙酯、O-磷酸-L-酪氨酸为磷源的菌藻共培养体系生长与产油情况较优。
Abstract:
Lipid production of microalgae has been widely concerned because of its clean and non-toxic characteristics. However, high cultivation cost and low lipid production efficiency have restricted its engineering application. To solve this problem, the effects of four kinds of phosphorus source including typical inorganic phosphorus, common organic phosphorus, nucleoside monophosphate and cyclic nucleoside monophosphate on the growth and lipid production of Chlorella pyrenoidesa in mono-algae culture and bacteria-algae co-culture system were investigated, and the number of algal cells growing, neutral lipid yield and changes of phosphorus and alkaline phosphatase contents in different culture systems were detected by ultraviolet analysis and Nile red staining. There was no significant difference in phosphorus utilization between the two systems. The number of algal cells (4.94×108 cells/10 μL) in the mono-algal culture system was higher than that in the co-culture system (3.38×108 cells/10 μL), but the lipid production in the co-culture system was higher. The growth and lipid production of the bacteria-algae co-culture system with phosphate, trimetaphosphate, triethyl phosphate and O-phospho-L-tyrosine as phosphorus sources was better.
更新日期/Last Update: 2023-04-01