[1]冯萱,陈宁,张欣,等.地塞米松胁迫下好氧颗粒污泥微观响应特征[J].中国给水排水,2023,39(19):1-10.
　FENGXuan,CHENNing,ZHANGXin,et al.Microscopic Response Characteristics of Aerobic Granular Sludge under Dexamethasone Stress[J].China Water & Wastewater,2023,39(19):1-10.
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地塞米松胁迫下好氧颗粒污泥微观响应特征

中国给水排水[ISSN:1000-4062/CN:12-1073/TU] 卷: 第39卷 期数: 2023年第19期 页码: 1-10 栏目: 出版日期: 2023-10-01
Title:
Microscopic Response Characteristics of Aerobic Granular Sludge under Dexamethasone Stress
作者:
冯萱1， 陈宁1， 张欣1， 马翠1， 刘崇2， 沈斐3， 王硕1,4， 李激1,4
1.江南大学环境与土木工程学院 江苏省厌氧生物技术重点实验室，江苏 无锡 214122；2.民政部环境监测中心站 民政部一零一研究所，北京 100070；3.江苏无锡环境监测中心 仪器分析实验室，江苏 无锡 214121；4.江苏高校水处理技术与材料协同创新中心，江苏 苏州 215009
Author(s):
FENG　Xuan1， CHEN　Ning1， ZHANG　Xin1， MA　Cui1， LIU　Chong2， SHEN　Fei3， WANG　Shuo1，4， LI　Ji1，4
（1. Jiangsu Key Laboratory of Anaerobic Biotechnology， School of Environment and Civil Engineering， Jiangnan University， Wuxi 214122， China； 2. 101 Institute of the Ministry of Civil Affairs， Environmental Monitoring Center Station of the Ministry of Civil Affairs， Beijing 100070， China； 3. Laboratory of Instrumental Analysis， Jiangsu Wuxi Environmental Monitoring Center， Wuxi 214121， China； 4. Jiangsu University Collaborative Innovation Center of Water Treatment Technology and Material， Suzhou 215009， China）
关键词:
好氧颗粒污泥;  地塞米松;  胞外聚合物;  蛋白质组学;  微观响应
Keywords:
aerobic granular sludge;  dexamethasone;  extracellular polymeric substances;  proteomics;  microscopic response
摘要:
研究了地塞米松胁迫条件下不同粒径好氧颗粒污泥（AGS）的物化特性和微观变化，并结合胞外聚合物（EPS）和蛋白质组学进一步解析AGS对地塞米松的微观响应机制。结果表明，AGS对COD、总氮和总磷的去除率稳定升高至88.3%、80.8%和76.1%，地塞米松的去除率也升高至55.2%。TB-EPS层的蛋白质和多糖含量均较高，SMP-EPS层及LB-EPS层含量较低，0.6~0.8 mm粒径范围的AGS中脯氨酸（Pro）相对丰度最高，说明此粒径的AGS能够更好地抵御地塞米松的胁迫，从而有利于AGS粒径稳定增大。色氨酸类蛋白质在0.6~0.8 mm的AGS中相对丰度较高，说明其耐药性较好。此外，粒径为0.6~0.8 mm和0.8~1 mm的AGS中α螺旋和β折叠结构占比相对较高，证明AGS稳定性相对较好。AGS内部发挥关键作用的KEGG通路为代谢途径，包括能量代谢、氨基酸代谢以及脂质代谢等。差异蛋白数量最多的AGS组别为大于2 mm和小于0.6 mm的AGS，说明粒径的差异越大，其内部优势微生物种群的差异也越大，相应的蛋白功能也存在明显差异。1~2 mm和0.8~1 mm的AGS蛋白质组学特征相差较小，但AGS内部代谢途径以及蛋白功能差别明显，结合PCA分析推测0.8~1 mm的AGS内部正经历关键的微生物种群演替。
Abstract:
The physicochemical properties and microscopic changes of AGS with different particle sizes under dexamethasone stress were investigated, and the microscopic response mechanism of AGS to dexamethasone was further analyzed based on EPS and proteomics. The experimental results showed that the removal of COD, total nitrogen and total phosphorus by AGS steadily increased to 88.3%, 80.8% and 76.1%, and dexamethasone removal increased up to 55.2%. The protein and polysaccharide contents of TB-EPS were higher, while the contents in SMP-EPS and LB-EPS were relatively lower. The relative abundance of proline (Pro) in AGS with the particle size of 0.6-0.8 mm was the highest, indicating that AGS with this particle size could better resist the stress of dexamethasone, which was conducive to the stable increase of AGS particle size. Tryptophan-like protein was relatively abundant in 0.6-0.8 mm AGS, implying that the dexamethasone resistance was good. In addition, the α?helix and β-sheet structures of proteins accounted for relatively high proportion in AGS with sizes of 0.6-0.8 mm and 0.8-1 mm, suggesting the relative stability of AGS. Through the analysis of KEGG pathway, metabolic pathway played a crucial role in AGS, including energy metabolism, amino acid metabolism and lipid metabolism. The AGS with the most differential proteins were those with the size greater than 2 mm and less than 0.6 mm, indicating that the greater the difference in particle size, the greater the difference in the internal dominant microbial population, and the corresponding protein functions also presented significant differences. Furthermore, in AGS of 1-2 mm and 0.8-1 mm, the difference in proteomic characteristics was small, however, the differences in internal metabolic pathways and protein functions were significant. On the basis of PCA analysis, it was speculated that 0.8-1 mm of AGS was undergoing critical microbial population succession.

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更新日期/Last Update: 2023-10-01